# US 9,752,173 B2 · Compositions and methods for inhibiting terminal transferase activity

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- Number: US 9,752,173 B2
- Document ID: US9752173B2
- Status: Granted
- Kind: B2
- Application number: 14/754,262
- Filed: 2015-06-29
- Published: 2015-12-31
- Granted: 2017-09-05
- Earliest claimed: 2010-04-08
- Assignees: Ibis Biosciences, Inc.
- Applicants: Ibis Biosciences, Inc.; Nina M. Hofstadler
- Inventors: Sherilynn Manalili Wheeler; James C. Hannis; Steven A. Hofstadler
- Web page: https://patentgrep.com/patent/US9752173B2

## CPC

- C12P 19/34: Polynucleotides, e.g. nucleic acids, oligoribonucleotides
- C07K 14/4703: {Inhibitors; Suppressors}
- C07K 14/5406: {IL-4}
- C12Q 1/686: Polymerase chain reaction [PCR]
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention realtes to systems and methods for amplifying nucleic acid. In particular, systems and methods are provided for inhibiting polymerase based terminal transferase activity within a polynucleotide amplification setting (e.g., polymerase chain reaction). In addition, systems and methods are provided for generating amplified products generated with polynucleotide amplification techniques having reduced 3′ non-templated nucleotide addition.

## Claims

### Claim 1

A kit comprising a PCR reaction solution, wherein said PCR reaction solution comprises boric acid, deoxynucleotide triphosphates, divalent cations, KCl and Tris-HCl or (NH)4SO4 and Tris-HCl, and a polymerase having terminal transferase activity.

### Claim 2 (depends on 1)

The kit of claim 1, wherein said divalent cations are selected from group consisting of Mg2+and Mn2+divalent cations.

### Claim 3 (depends on 1)

The kit of claim 1, wherein said polymerase having terminal transferase activity is Taq polymerase or a fragment thereof.