Stable expression system for eukaryotic cells
AssigneeBiogenomics Limited
InventorsArchana Rajesh Krishnan, Sanjay Madhukar Sonar, Damodar Krishnabahadur Thappa
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Abstract
The present invention related to a polynucleotide sequence and an expression vector comprising at least one gene encoding a stress resistance protein, at least one gene encoding a selection marker, at least one gene encoding an expression protein, at least one matrix attachment region and a transcription terminator, all of which are operably connected to each other. The present invention further relates to a host cell comprising the expression vector. The present invention also relates a method of producing a cell line.
Claims10
An expression vector for expressing a recombinant protein in a mammalian cell, the vector comprising:
- in the following operative order, a first promoter, a first sequence coding for a stress resistance protein, an internal ribosome entry site (IRES), and a second sequence coding for a selection marker, wherein the first and second sequences are arranged to allow transcription of a single mRNA from the first promoter that encodes the stress resistance protein and the selection marker;
- a matrix attachment region (MAR);
- and
- a second promoter operably linked to multiple cloning site for inserting a third sequence coding for the recombinant protein.
Dependencies are inferred from the claim text.