# US 9,752,148 B2 · Extraction, preparation, and application of plant micro-ribonucleic acid

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- Number: US 9,752,148 B2
- Document ID: US9752148B2
- Status: Granted
- Kind: B2
- Application number: 14/422,117
- Filed: 2013-08-15
- Published: 2015-10-15
- Granted: 2017-09-05
- Earliest claimed: 2012-08-15
- Assignees: Micromedmark Biotech Co., Ltd.
- Applicants: Micromedmark Biotech Co., Ltd.
- Inventors: Chenyu Zhang; Junfeng Zhang; Ke Zeng; Lei Dong
- Web page: https://patentgrep.com/patent/US9752148B2

## CPC

- C12N 15/1131: {against viruses}
- A61K 31/7105: Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
- A61K 31/713: Double-stranded nucleic acids or oligonucleotides
- A61K 36/35: Caprifoliaceae (Honeysuckle family)
- A61K 36/355: Lonicera (honeysuckle)
- A61P 1/00: Drugs for disorders of the alimentary tract or the digestive system
- A61P 3/00: Drugs for disorders of the metabolism (of the blood or the extracellular fluid A61P7/00)
- A61P 5/00: Drugs for disorders of the endocrine system
- A61P 7/00: Drugs for disorders of the blood or the extracellular fluid
- A61P 9/00: Drugs for disorders of the cardiovascular system
- A61P 11/00: Drugs for disorders of the respiratory system
- A61P 13/00: Drugs for disorders of the urinary system
- A61P 15/00: Drugs for genital or sexual disorders (for disorders of sex hormones A61P5/24); Contraceptives
- A61P 19/00: Drugs for skeletal disorders
- A61P 25/00: Drugs for disorders of the nervous system
- A61P 31/00: Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P 31/12: Antivirals
- A61P 31/16: for influenza or rhinoviruses
- A61P 35/00: Antineoplastic agents
- A61P 37/00: Drugs for immunological or allergic disorders
- C12N 15/113: Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)}
- C12N 15/8218: {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]}
- C12Q 1/6895: for plants, fungi or algae
- C12N 2310/113: targeting other non-coding nucleic acids, e.g. antagomirs
- C12N 2310/141: MicroRNAs, miRNAs
- C12N 2320/30: Special therapeutic applications
- C12Q 2600/178: miRNA, siRNA or ncRNA
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

An isolated plant-functional microRNA or an extract containing said plant-functional microRNA, a preparation method therefor, and an application thereof are provided. The plant-functional microRNA originates from the endogenous microRNA of a plant, is present in a water-soluble and/or fat-soluble extract of said plant, and is capable of regulating non-plant target-genes.

## Claims

### Claim 1

A method for preparing a pharmaceutical comprising:
extracting MIR2911 from a plant using water to obtain an extract containing MIR2911; and
mixing the extract containing MIR2911 with a carrier acceptable in pharmaceutics to form said pharmaceutical,
wherein the extract containing MIR2911 is a water extract of Lonicera japonica
and contains 0.01-100 nM MIR2911, and
the extract containing MIR2911 has a MIR2911 content that is greater than or equal to 70% of a total amount of microRNAs in the extract containing MIR2911.

### Claim 2 (depends on 1)

The method of claim 1, wherein said extract containing MIR2911 contains 0.1-20 nM MIR2911.

### Claim 3 (depends on 1)

The method of claim 1, wherein the extract containing MIR2911 is an aged extract obtained from an aging treatment.

### Claim 4 (depends on 3)

The method of claim 3, wherein the aging treatment is a placement.

### Claim 5 (depends on 3)

The method of claim 3, wherein the aging treatment is a placement at 20° C.-50° C. for 2 hours to 10 days.

### Claim 6

A method for treating a viral cold comprises administering a microRNA molecule MIR2911 at a concentration of 0.01-100 nM to a subject in need thereof.

### Claim 7

A method for treating a viral cold comprises administering a pharmaceutical comprising an extract containing MIR2911 and a carrier acceptable in pharmaceutics to a subject in need thereof,
wherein the extract containing MIR2911 is an extract of Lonicera japonica,
the extract containing MIR2911 is a water-soluble extract of plants, and
the extract containing MIR2911 contains 0.01-100 nM MIR2911.

### Claim 8 (depends on 7)

The method of claim 7, wherein the extract containing MIR2911 is an aged extract obtained by aging treatment.

### Claim 9 (depends on 8)

The method of claim 8, wherein the aging treatment is a placement.

### Claim 10 (depends on 8)

The method of claim 8, wherein the aging treatment is a placement at 20° C.-50° C. for 2 hours to 10 days.

### Claim 11 (depends on 1)

The method of claim 1, wherein the carrier is one or more of cellulose or a derivative thereof, gelatin, talc, solid lubricant, calcium sulfate, vegetable oil, polyol, an emulsifying agent, a wetting agent, a colorant, a flavoring agent, a stabilizing agent, an antioxidant, and a preservative.