# US 9,752,120 B2 · Activated platelet composition with tunable growth factor level

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- Number: US 9,752,120 B2
- Document ID: US9752120B2
- Status: Granted
- Kind: B2
- Application number: 14/674,971
- Filed: 2015-03-31
- Published: 2016-10-06
- Granted: 2017-09-05
- Earliest claimed: 2015-03-31
- Assignees: General Electric Company
- Applicants: General Electric Company
- Inventors: Vasile Bogdan Neculaes; Juan Jose Becerra; Christine Angela Morton; Andrew Soliz Torres; Antonio Caiafa
- Web page: https://patentgrep.com/patent/US9752120B2

## CPC

- C12N 5/0644: {Platelets; Megakaryocytes}
- C12M 1/36: including condition or time responsive control, e.g. automatically controlled fermentors (controlling or regulating in general G05)
- C12M 1/42: Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
- C12M 35/02: {Electrical or electromagnetic means, e.g. for electroporation or for cell fusion}
- C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)}
- C12N 2529/00: Culture process characterised by the use of electromagnetic stimulation
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

Methods and systems for generating a tunable or customizable activated product composition are related. In certain embodiments, one or more of electric pulse parameters, flow rate, or sample container size are varied so as to generate the activated product composition. The activated product composition may be customized or optimized based for a particular patient or procedure.

## Claims

### Claim 1

A method for releasing growth factors, comprising:
positioning a sample with respect to electrodes of a pulse generating apparatus;
wherein the sample comprises one of a platelet rich plasma sample, a platelet suspension, or a whole blood sample;
specifying a set of electric pulse parameters, wherein different parameter values yield different levels of at least one growth factor in an activated product composition;
wherein the at least one growth factor comprise growth factors released by one or more of platelets, red blood cells, or white blood cells, including one or more of platelet derived growth factor, epidermal growth factor, vascular endothelial growth factor, or transforming growth factor beta 1 and wherein the level of at least one growth factor of the activated product composition is substantially equal to or larger than a level obtained using a thrombin activation; and
exposing the sample to one or more electric pulses generated in accordance with the parameter values, wherein the sample, when exposed to the one or more electric pulses, yields the activated product composition having levels for the at least one growth factor adjustably controlled at least in part by the set of electric pulse parameters.

### Claim 2 (depends on 1)

The method of claim 1, further comprising:
selecting a container or conduit size from a plurality of container sizes; and
placing the sample within the container having the selected size,
wherein positioning the sample comprises placing the container holding the sample within a sample holder between the electrodes, wherein the activated product composition has levels of at least one growth factor determined at least in part by the container size.

### Claim 3 (depends on 1)

The method of claim 1, wherein positioning the sample between the electrodes comprises flowing the sample through a conduit between the electrodes, wherein the activated product composition has levels of the at least one growth factor determined at least in part by one or both of the conduit diameter or the flow rate of the sample through the conduit.

### Claim 4 (depends on 1)

The method of claim 1, wherein the set of electric pulse parameters comprise one or more of a voltage, an electric field, a current, a pulse width, an energy density, energy per platelet, or a number of pulses.

### Claim 5 (depends on 1)

The method of claim 1, wherein the sample comprise cells that are not lysed in response to the one or more electric pulses.

### Claim 6

A method for releasing growth factors, comprising:
selecting a cuvette size from among a plurality of cuvette sizes, wherein different cuvette sizes of the plurality yield different levels of one or more growth factors in an activated product composition;
placing a sample within a cuvette of the selected size;
wherein the sample comprises one of a platelet rich plasma sample, a platelet suspension, or a whole blood sample;
placing the cuvette within a sample holder of a pulse generating apparatus;
exposing the sample to one or more electric pulses, wherein the sample, when exposed to the one or more electric pulses, yields the activated product composition having levels of the one or more growth factors adjustably controlled at least in part by the cuvette size;
wherein the one or more growth factors comprise growth factors released by one or more of platelets, red blood cells, or white blood cells, including one or more of platelet derived growth factor, epidermal growth factor, vascular endothelial growth factor, or transforming growth factor beta 1; and
wherein the level of the at least one growth factor of the activated product composition is substantially equal to or larger than a level obtained using a thrombin activation.

### Claim 7 (depends on 6)

The method of claim 6, further comprising:
specifying a set of electric pulse parameters, wherein different parameter values yield different levels of one or more growth factors in the activated product composition, and wherein the activated product composition has levels of the one or more growth factor determined at least in part by the set of electric pulse parameters.

### Claim 8 (depends on 7)

The method of claim 7, wherein the set of electric pulse parameters comprise one or more of a voltage, an electric field, a current, a pulse width, an energy density, or a number of pulses.

### Claim 9 (depends on 6)

The method of claim 6, wherein the sample comprises cells that are not lysed in response to the one or more electric pulses.

### Claim 10

A method for customizing an activated blood-derived cell treatment, comprising:
adjustably controlling a customized growth factor profile for treating a patient based on one or both of type of wound or a respective process of the wound healing cascade;
wherein adjustably controlling the customized growth factor profile comprises adjustably controlling an amount of one or more growth factors to be present in the activated product composition relative to an amount of one or more different growth factors to be present in the activated product composition;
wherein the growth factors consist of platelet derived growth factor, epidermal growth factor, vascular endothelial growth factor and transforming growth factor beta 1;
selecting one or more electric pulse parameters and a cuvette size corresponding to the adjustably controlled growth factor profile;
exposing a sample placed in a cuvette of the selected size to one or more electric pulses generated based on the selected electric pulse parameters;
wherein the sample comprises one of a platelet rich plasma sample, a platelet suspension, or a whole blood sample; and
generating an activated product composition having the customized growth factor profile.

### Claim 11 (depends on 10)

The method of claim 10, wherein selecting the one or more electric pulse parameters comprises specifying values for one or more of a voltage, an electric field, a current, a pulse width, an energy density, or a number of pulses.

### Claim 12 (depends on 10)

The method of claim 10, wherein selecting the one or more electric pulse parameters comprises selecting a preconfigured pulse profile from a plurality of preconfigured pulse profiles.