# US 9,752,117 B2 · Hybrid tissue scaffold for tissue engineering

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- Number: US 9,752,117 B2
- Document ID: US9752117B2
- Status: Granted
- Kind: B2
- Application number: 14/467,879
- Filed: 2014-08-25
- Published: 2015-02-19
- Granted: 2017-09-05
- Earliest claimed: 2012-12-12
- Assignees: Southwest Research Institute
- Applicants: Southwest Research Institute
- Inventors: Jeffrey N. Harris; Jian Ling; Xingguo Cheng
- Web page: https://patentgrep.com/patent/US9752117B2

## CPC

- C12N 5/0068: {General culture methods using substrates (for specific animal cell type C12N5/06)}
- A61L 27/18: obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- A61L 27/24: Collagen
- A61L 27/26: Mixtures of macromolecular compounds
- A61L 27/38: {containing added animal cells (organs or tissue containing native cells A61L27/36)}
- A61L 27/56: Porous materials, {e.g. foams or sponges}
- A61L 2400/06: Flowable or injectable implant compositions
- C12N 2533/30: Synthetic polymers
- C12N 2533/50: Proteins
- C12N 2533/54: Collagen; Gelatin
- C12N 2533/56: Fibrin; Thrombin
- C12N 2535/00: Supports or coatings for cell culture characterised by topography
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)

## Abstract

A hybrid tissue scaffold is provided which comprises a porous primary scaffold having a plurality of pores and a porous secondary scaffold having a plurality of pores, wherein the secondary scaffold resides in the pores of the primary scaffold to provide a hybrid scaffold. The pores of the porous primary scaffold may have a pore size in a range of 0.50 mm to 5.0 mm, and the pores of the porous secondary scaffold may have a pore size in a range of 50 μm to 600 μm. The primary scaffold may provide 5% to 30% of a volume of the hybrid scaffold.

## Claims

### Claim 1

A hybrid tissue scaffold comprising:
a porous primary scaffold of biodegradable polymer having pores in a pore size range of 1.0 mm to 4.0 mm;
a porous secondary scaffold formed from a biomaterial injectable into the pores of the porous primary scaffold, the biomaterial comprising a hydrated hydrogel including living cells, wherein the secondary scaffold resides in the pores of the primary scaffold to provide a hybrid scaffold;
wherein the biomaterial provides an environment to culture the living cells and the biomaterial has a pore size range of 50 μm to 600 μm; and
wherein the primary scaffold provides 5% to 30% of a volume of the hybrid scaffold and said hybrid scaffold has a Young's modulus of 0.1 MPA to 100 GPa.

### Claim 2 (depends on 1)

The tissue scaffold of claim 1 wherein:
the pores of the porous secondary scaffold have a pore size in a range of 200 μm to 500 μm.

### Claim 3 (depends on 1)

The tissue scaffold of claim 1 wherein:
the porous primary biodegradable scaffold comprises a synthetic polymer.

### Claim 4 (depends on 3)

The tissue scaffold of claim 3 wherein:
the synthetic polymer is a polyester.

### Claim 5 (depends on 1)

The tissue scaffold of claim 1 wherein:
the biomaterial further comprises a naturally occurring polymer.

### Claim 6 (depends on 5)

The tissue scaffold of claim 5 wherein:
the naturally occurring polymer is a protein.

### Claim 7 (depends on 6)

The tissue scaffold of claim 6 wherein:
the protein is collagen.

### Claim 8 (depends on 6)

The tissue scaffold of claim 6 wherein:
the protein is fibrin.

### Claim 9 (depends on 5)

The tissue scaffold of claim 5 wherein:
the naturally occurring polymer is a carbohydrate.

### Claim 10 (depends on 1)

The tissue scaffold of claim 1 wherein:
the cells comprise at least one of endothelial, fibroblast and stem cells.

### Claim 11 (depends on 1)

The tissue scaffold of claim 1 wherein:
the biomaterial does not contain any crosslinking.

### Claim 12 (depends on 1)

The tissue scaffold of claim 1 wherein:
the primary scaffold is provides 10.0% to 20.0% of the volume of the hybrid scaffold.

### Claim 13 (depends on 1)

The tissue scaffold of claim 1 wherein:
the biomaterial has a porosity of 50% to 99% by volume.