# US 9,751,956 B2 · Method of using supercritical fluid technology to separate and purify functional components of Antrodia camphorata

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- Number: US 9,751,956 B2
- Document ID: US9751956B2
- Status: Granted
- Kind: B2
- Application number: 14/828,658
- Filed: 2015-08-18
- Published: 2016-09-08
- Granted: 2017-09-05
- Earliest claimed: 2015-03-06
- Assignees: Ultra-Microrigin Biomedical Technology Co., Ltd.
- Applicants: Ultra-Microrigin Biomedical Technology Co., Ltd.
- Inventors: Ming-Hsi Chuang; Chu-Ting Lin; I-Lung Yu; Lin-Hsiang Chuang
- Web page: https://patentgrep.com/patent/US9751956B2

## CPC

- C08B 37/0003: {General processes for their isolation or fractionation, e.g. purification or extraction from biomass}
- C07J 9/005: {containing a carboxylic function directly attached or attached by a chain containing only carbon atoms to the cyclopenta[a]hydrophenanthrene skeleton}
- C07J 63/008: {Expansion of ring D by one atom, e.g. D homo steroids}
- Y02P 20/54: using solvents, e.g. supercritical solvents or ionic liquids
- C08B: POLYSACCHARIDES; DERIVATIVES THEREOF (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages C07H; fermentation or enzyme-using processes C12P19/00; sugar industry C13; production of cellulose D21)
- C07J: STEROIDS (seco-steroids C07C)
- Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

## Abstract

A method of using supercritical fluid technology to separate and purify the functional components of Antrodia cinnamomea is described. Triterpenoids and polysaccharides of Antrodia cinnamomea are extracted from a fractionation tank containing extract of Antrodia cinnamomea and supercritical solvent at a set temperature and pressure. The extracted functional components and supercritical solvent are then continuously fed into three separating tanks at a set temperature and a pressure below that of the aforesaid pressure to obtain various functional components of triterpenoids and polysaccharides from the Antrodia cinnamomea feed in each separating tank.

## Claims

### Claim 1

A method to separate and purify functional components of Antrodia cinnamomea comprising:
mixing Antrodia cinnamomea powder and ethanol in a ratio of 1:5 to 10 (weight per volume);
centrifuging the mixture of Antrodia cinnamomea powder and ethanol so as to obtain a Antrodia cinnamome feed;
introducing the Antrodia cinnamome feed at a flow velocity of 1 L/hr to 3 L/hr into a fractionating tank at pressures of 2000 psi to 4000 psi and temperatures of 40° C. to 60° C. with a supercritical solvent of carbon dioxide/ethanol at a fluid flow velocity of 3 L/hr to 9 L/hr so as to extract triterpenoids of Antrodia cinnamomea from a top of the fractionating tank and polysaccharides of Antrodia cinnamomea from a bottom of the fractionating tank; and
conveying the extracts of Antrodia cinnamomea and supercritical solvent continuously into three separating tanks at a temperature of 40° C. or 60° C. and reducing pressure stepwise from 3000 psi to 2000 psi and to 1000 psi so as to selectively increase the content and purity of the functional components of triterpenoids and polysaccharides and separate the solvent in a supercritical state.

### Claim 2 (depends on 1)

The method defined in claim 1, wherein the Antrodia cinnamomea powder is the powder of the fruiting body, mycelium, dish culture, or solid culture of Antrodia cinnamomea.

### Claim 3 (depends on 1)

The method defined in claim 1, wherein, under the operating conditions in the fractionating tank of a temperature set at 40° C. and a pressure set at 4000 psi, the selectivity of triterpenoids and polysaccharides of Antrodia cinnamomea is 220.