Antibodies to risperidone haptens and use thereof
AssigneeJanssen Pharmaceutica NV
InventorsEric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Maarten Vliegen, Pieter Rik Haspeslagh
Life of this patent
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Abstract
Disclosed is an antibody which binds to risperidone, which can be used to detect risperidone in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of risperidone, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
Claims8
A method of producing an antibody which binds to risperidone, the method comprising:
- (i) selecting a host for antibody production;
- and
- (ii) inoculating the host with a conjugate of a compound of Formula I and an immunogenic carrier, wherein the host produces the antibody which binds to risperidone,
- Formula I:
- wherein:
- R1 is H, or OH;
- R2 is
- or O(CH2)rNHC(O)(CH2)mCO2H;
- wherein:
- r is 1, 2, 3, 4, or 5;
- m is 1, 2, 3, 4, or 5;
- and
- n is 1, 2, 3, 4, or 5.
A method of producing a hybridoma cell line, which produces a monoclonal antibody which binds to risperidone, the method comprising:
- (i) selecting a host for antibody production;
- (ii) inoculating the host with a conjugate of a compound of Formula I and an immunogenic carrier;
- (iii) fusing a cell line from said inoculated host, wherein the cell line produces antibodies that bind to risperidone, with a continuously dividing cell to create a fused cell, which produces a monoclonal antibody which binds to risperidone;
- and
- (iv) cloning the fused cell so as to obtain a hybridoma cell line,
- Formula I:
- wherein:
- R1 is H, or OH;
- R2 is
- or O(CH2)rNHC(O)(CH2)mCO2H;
- wherein:
- r is 1, 2, 3, 4, or 5;
- m is 1, 2, 3, 4, or 5;
- and
- n is 1, 2, 3, 4, or 5.
Dependencies are inferred from the claim text.