# US 9,751,941 B2 · Antigen derived from extracellular domain of multi-transmembrane protein and uses thereof

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- Number: US 9,751,941 B2
- Document ID: US9751941B2
- Status: Granted
- Kind: B2
- Application number: 13/803,090
- Filed: 2013-03-14
- Published: 2014-09-18
- Granted: 2017-09-05
- Earliest claimed: 2013-03-14
- Assignees: Abion Inc.
- Applicants: REFERENCE BIOLABS Inc.
- Inventors: Young Kee Shin; Young-Deug Kim; Jun Young Choi; Myung Seok Lee
- Web page: https://patentgrep.com/patent/US9751941B2

## CPC

- C07K 16/28: against receptors, cell surface antigens or cell surface determinants
- C07K 2317/24: containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
- C07K 2317/34: Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
- C07K 2317/622: Single chain antibody (scFv)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

A multi-transmembrane protein antigen includes a polypeptide corresponding to an extracellular loop of the multi-transmembrane protein, the N-terminal and C-terminal of the polypeptide being fixed on a solid substrate or the N-terminal and C-terminal being attached to both ends of a linker to form a cyclic structure, an antibody specifically binding to the antigen or an antigen-binding fragment thereof, and a method for screening an antibody specifically binding to the antigen. The present invention may be usefully employed for effective production of antibodies for multi-transmembrane proteins that play important roles in disease-related phenomena such as cell signaling.

## Claims

### Claim 1

A multi-transmembrane protein antigen comprising a polypeptide corresponding to an extracellular loop of a multi-transmembrane protein, wherein an N-terminal and a C-terminal of the polypeptide are fixed on a solid substrate, or an N-terminal and a C-terminal of the polypeptide are attached to both ends of a linker to form a cyclic structure, wherein polypeptide corresponding to the extracellular loop of the multi-transmembrane protein is a polypeptide consisting of the amino acid sequence of SEQ ID NO: 3 or 4.

### Claim 2 (depends on 1)

The antigen according to claim 1, wherein the N-terminal and the C-terminal of the polypeptide are fixed on the solid substrate.

### Claim 3 (depends on 1)

The antigen according to claim 1, wherein the N-terminal and the C-terminal of the polypeptide are attached to both ends of the linker to form the cyclic structure.

### Claim 4 (depends on 3)

The antigen according to claim 3, wherein the linker is selected from the group consisting of aminohexanoic acid, polyglycine and polyalanine.

### Claim 5 (depends on 3)

The antigen according to claim 3, wherein the linker is attached to a water-soluble resin.

### Claim 6 (depends on 5)

The antigen according to claim 5, wherein the water-soluble resin is enclosed by polyethylene glycol (PEG).