# US 9,751,918 B2 · Transgenic plants expressing cobalamin binding proteins

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 9,751,918 B2
- Document ID: US9751918B2
- Status: Granted
- Kind: B2
- Application number: 12/357,622
- Filed: 2009-01-22
- Published: 2010-07-22
- Granted: 2017-09-05
- Earliest claimed: 2001-07-13
- Assignees: Xeragenx LLC
- Applicants: Lars Erik Berglund; Torben Ellebaek Petersen; Sergey Nikolaevich Fedosov; Ebba Nexo; Niels Bech Laursen; Erik Ostergaard Jensen
- Inventors: Lars Erik Berglund; Torben Ellebaek Petersen; Sergey Nikolaevich Fedosov; Ebba Nexo; Niels Bech Laursen; Erik Ostergaard Jensen
- Web page: https://patentgrep.com/patent/US9751918B2

## CPC

- C07K 14/47: from mammals
- A61K 31/7056: containing five-membered rings with nitrogen as a ring hetero atom
- A61K 38/168: {from plants}
- A61K 47/551: {one of the codrug's components being a vitamin, e.g. niacinamide, vitamin B3, cobalamin, vitamin B12, folate, vitamin A or retinoic acid}
- A61P 3/02: Nutrients, e.g. vitamins, minerals
- C07K 14/415: from plants
- C12N 5/0602: {Vertebrate cells}
- C12N 15/8257: {for the production of primary gene products, e.g. pharmaceutical products, interferon}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- C12N 2500/38: Vitamins
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to the use of transgenic plants for the expression of vitamin B12 (cobalamin) binding proteins. Plant cells are transformed with nucleotide sequences adapted for expression and secretion of vitamin B12 binding proteins. The present invention also relates to the use of recombinant vitamin B12 binding proteins from plants in analytical tests and treatment of vitamin B12 deficiency. Also disclosed is a method for purification of recombinant vitamin B12 binding proteins.

## Claims

### Claim 1

A composition, the composition comprising intrinsic factor and a plant specific carbohydrate chain on the intrinsic factor, wherein all of the intrinsic factor is in the apo form.