# US 9,751,758 B2 · Rational assembly of nanoparticle superlattices with designed lattice symmetries > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,751,758 B2 - Document ID: US9751758B2 - Status: Granted - Kind: B2 - Application number: 14/373,161 - Filed: 2013-01-18 - Published: 2015-01-15 - Granted: 2017-09-05 - Earliest claimed: 2012-01-18 - Assignees: Brookhaven Science Associates, LLC - Applicants: Brookhaven Science Associates, LLC - Inventors: Oleg Gang; Fang Lu; Miho Tagawa - Web page: https://patentgrep.com/patent/US9751758B2 ## CPC - B82B 3/00: Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units - C12Q 1/68: involving nucleic acids - B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites - B82Y 40/00: Manufacture or treatment of nanostructures - Y10S 977/84: Manufacture, treatment, or detection of nanostructure - Y10T 428/2982: Particulate matter [e.g., sphere, flake, etc.] - B82B: NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS, MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES AS DISCRETE UNITS; MANUFACTURE OR TREATMENT THEREOF - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES - Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract A method for lattice design via multivalent linkers (LDML) is disclosed that introduces a rationally designed symmetry of connections between particles in order to achieve control over the morphology of their assembly. The method affords the inclusion of different programmable interactions within one linker that allow an assembly of different types of particles. The designed symmetry of connections is preferably provided utilizing DNA encoding. The linkers may include fabricated “patchy” particles, DNA scaffold constructs and Y-shaped DNA linkers, anisotropic particles, which are preferably functionalized with DNA, multimeric protein-DNA complexes, and particles with finite numbers of DNA linkers. ## Claims ### Claim 1 A controllable nanoparticle superlattice comprising, a linker comprising a specified symmetry of connecting sites, wherein the linker is a symmetric DNA scaffold construct, an anisotropic particle, a multimeric protein-DNA complex, or a “patchy” particle, and wherein the connecting sites of the linker have non-complementary DNA and/or RNA attached thereto; and a nanoparticle encapsulated with non-complementary DNA and/or RNA, wherein the non-complementary DNA and/or RNA of the nano particle is complementary to the non-complementary DNA and/or RNA of the linker so as to form a nanoparticle superlattice, wherein the symmetry of the superlattice is determined by the specified symmetry of the connecting sites of the linker. ### Claim 2 (depends on 1) The controllable nanoparticle superlattice according to claim 1, wherein the linker is a symmetric DNA scaffold construct or an anisotropic particle. ### Claim 3 (depends on 1) The controllable nanoparticle superlattice according to claim 1, wherein the the linker is a symmetric DNA scaffold construct. ### Claim 4 (depends on 3) The controllable nanoparticle superlattice according to claim 3, wherein the symmetric DNA scaffold construct has one of a cubic geometry, tetrahedron geometry, or octahedron geometry. ### Claim 5 (depends on 4) The controllable nanoparticle superlattice according to claim 4, wherein the symmetric DNA scaffold construct is a DNA tetrahedron. ### Claim 6 (depends on 1) The controllable nanoparticle superlattice according to claim 1, wherein the formed nanoparticle superlattice is a diamond lattice, simple cubic lattice or perovskite lattice. ### Claim 7 (depends on 1) The controllable nanoparticle superlattice according to claim 1, wherein the anisotropic particle has connecting sites at particle facets. ### Claim 8 (depends on 1) The controllable nanoparticle superlattice according to claim 1, wherein the anisotropic particle has connecting sites at particle vertices. ### Claim 9 (depends on 1) The controllable nanoparticle superlattice according to claim 1, wherein the anisotropic particle is metal. ### Claim 10 (depends on 9) The controllable nanoparticle superlattice according to claim 9, wherein the metal is selected from the group consisting of gold, silver, and platinum. ### Claim 11 (depends on 1) The controllable nanoparticle superlattice according to claim 1, wherein the linker is an anisotropic particle comprising a semiconductor. ### Claim 12 (depends on 11) The controllable nanoparticle superlattice according to claim 11, wherein the semiconductor is selected from the group consisting of cadmium selenide, cadmium sulfide, zinc sulfide, and gallium arsenide. ### Claim 13 (depends on 1) The controllable nanoparticle superlattice according to claim 1, wherein the linker is a magnetic anisotropic particle. ### Claim 14 (depends on 13) The controllable nanoparticle superlattice according to claim 13, wherein the magnetic anisotropic particle is iron oxide. ### Claim 15 (depends on 1) The controllable nanoparticle superlattice according to claim 1, wherein the linker is an anisotropic particle comprising silicon dioxide. ### Claim 16 (depends on 1) The controllable nanoparticle superlattice according to claim 1, wherein the linker is a multimeric protein-DNA complex comprising a knob adenovirus protein or streptavidin tetramer.