# US 9,751,082 B1 · Multi-actuated micro-pipette controller and associated use thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,751,082 B1 - Document ID: US9751082B1 - Status: Granted - Kind: B1 - Application number: 14/720,699 - Filed: 2015-05-22 - Granted: 2017-09-05 - Earliest claimed: 2014-05-22 - Applicants: Dunsong Yang - Inventors: Dunsong Yang - Web page: https://patentgrep.com/patent/US9751082B1 ## CPC - B01L 3/021: {Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids} - A61B 17/43: for artificial insemination - A61B 2017/00424: {ergonomic, e.g. fitting in fist} - B01L 3/0275: {Interchangeable or disposable dispensing tips} - B01L 2300/0838: Capillaries - B01L 2400/0481: squeezing of channels or chambers - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) ## Abstract A multi-actuated micro-pipette controller is actuated in at least three different ways. First, the multi-actuated micro-pipette controller may be employed similar to a striper for denudation of cumulus cells and transfer of oocytes and embryos by depressing an actuator assembly operably coupled to a ballonet. Second, the multi-actuated micro-pipette controller may be actuated similar to a SWEMED™ denudation pipette by pressing directly on the ballonet while it is seated in a primary housing and a secondary housing, without employing an actuator assembly of the primary housing. Third, the multi-actuated micro-pipette controller may be actuated by removing the ballonet and employing it similar to a PASTEUR™ pipette. ## Claims ### Claim 1 A multi-actuated micro-pipette controller for selectively transporting a volume of fluid, said multi-actuated micro-pipette controller comprising: a primary housing including an open proximal end and an open distal end axially opposed therefrom, a primary access port formed adjacent to said distal end, and a chamber extending from said open proximal end to said distal end such that said chamber is accessible from said primary access port; a secondary housing removably seated within said chamber, said secondary housing having a secondary access port at least partially aligned with said primary access port; a ballonet adapted to receive the fluid therein, said ballonet being removably inserted within said secondary housing and at least partially aligned with said secondary access port such that said ballonet is directly accessible and squeezable via said primary access port and said secondary access port; and an actuator assembly located at said primary housing and being operably engaged with said ballonet; wherein linear actuation of said actuator assembly compresses at least a portion of said ballonet and thereby causes the fluid to expel outwardly from said ballonet; wherein said primary housing has a centrally registered longitudinal axis axially aligned with said open proximal end and said open distal end, said primary access port and said secondary access port being axially offset from the centrally registered longitudinal axis; wherein said secondary housing is located adjacent to said open distal end of said primary housing and oppositely spaced from said open distal end of said primary housing; wherein said ballonet is oppositely spaced from said open proximal end of said primary housing. ### Claim 2 (depends on 1) The multi-actuated micro-pipette controller of claim 1, wherein said secondary housing is linearly inserted and retracted from said chamber of said primary housing during non-operating conditions. ### Claim 3 (depends on 1) The multi-actuated micro-pipette controller of claim 1, wherein said secondary housing remains statically nested within said chamber of said primary housing while said ballonet is compressed and released between tension and equilibrium positions, respectively. ### Claim 4 (depends on 1) The multi-actuated micro-pipette controller of claim 1, further comprising: a micro-pipette tip removably inserted into said open distal end of said primary housing and being in fluid communication with said ballonet. ### Claim 5 (depends on 1) The multi-actuated micro-pipette controller of claim 1, wherein, when said ballonet and said secondary housing are operably removed from said chamber of said primary housing, said ballonet and said secondary housing are operably disengaged from said actuator assembly such that the fluid is discharged outwardly away from said secondary housing by directly squeezing said ballonet positioned exterior of said primary housing. ### Claim 6 (depends on 1) The multi-actuated micro-pipette controller of claim 1, wherein, when said ballonet is operably removed from said secondary housing, the fluid is discharged outwardly away from said ballonet by directly squeezing said ballonet positioned exterior of both said primary housing and said secondary housing when said secondary housing is removed from said primary housing. ### Claim 7 (depends on 1) The multi-actuated micro-pipette controller of claim 1, wherein said primary access port and said secondary access port overlap along the longitudinal length of said primary housing. ### Claim 8 (depends on 1) The multi-actuated micro-pipette controller of claim 1, wherein said secondary housing remains spaced from said actuator assembly while said secondary housing and said ballonet are seated within said chamber of said primary housing. ### Claim 9 (depends on 1) The multi-actuated micro-pipette controller of claim 1, wherein said actuator assembly and said ballonet are oriented at an end-to-end configuration while seated within said chamber of said primary housing. ### Claim 10 A multi-actuated micro-pipette controller for selectively transporting a volume of fluid, said multi-actuated micro-pipette controller comprising: a primary housing including a proximal end and a distal end axially opposed therefrom, a primary access port formed adjacent to said distal end, and a chamber extending from said proximal end to said distal end such that said chamber is accessible from said primary access port; a secondary housing removably seated within said chamber, said secondary housing having a secondary access port at least partially aligned with said primary access port; a ballonet adapted to receive the fluid therein, said ballonet being removably inserted within said secondary housing and at least partially aligned with said secondary access port such that said ballonet is directly accessible and squeezable via said primary access port and said secondary access port; and an actuator assembly located at said primary housing and being operably engaged with said ballonet; wherein linear actuation of said actuator assembly compresses at least a portion of said ballonet and thereby causes the fluid to expel outwardly from said ballonet; wherein said secondary housing is coaxially aligned with said primary housing while said secondary housing is disposed within said chamber; wherein each of said primary access port and said second access port is spaced from said proximal end and said distal end of said primary housing. ### Claim 11 (depends on 10) The multi-actuated micro-pipette controller of claim 10, wherein said secondary housing is linearly inserted and retracted from said chamber of said primary housing during non-operating conditions. ### Claim 12 (depends on 11) The multi-actuated micro-pipette controller of claim 11, wherein said secondary housing remains statically nested within said chamber of said primary housing while said ballonet is compressed and released between tension and equilibrium positions, respectively. ### Claim 13 (depends on 12) The multi-actuated micro-pipette controller of claim 12, further comprising: a micro-pipette tip removably inserted into said distal end of said primary housing and being in fluid communication with said ballonet. ### Claim 14 (depends on 10) The multi-actuated micro-pipette controller of claim 10, wherein, when said ballonet and said secondary housing are operably removed from said chamber of said primary housing, said ballonet and said secondary housing are operably disengaged from said actuator assembly such that the fluid is discharged outwardly away from said secondary housing by directly squeezing said ballonet positioned exterior of said primary housing. ### Claim 15 (depends on 10) The multi-actuated micro-pipette controller of claim 10, wherein, when said ballonet is operably removed from said secondary housing, the fluid is discharged outwardly away from said ballonet by directly squeezing said ballonet positioned exterior of both said primary housing and said secondary housing when said secondary housing is removed from said primary housing. ### Claim 16 (depends on 13) The multi-actuated micro-pipette controller of claim 13, wherein said primary access port and said secondary access port overlap along the longitudinal length of said primary housing. ### Claim 17 (depends on 16) The multi-actuated micro-pipette controller of claim 16, wherein said secondary housing remains spaced from said actuator assembly while said secondary housing and said ballonet are seated within said chamber of said primary housing. ### Claim 18 (depends on 17) The multi-actuated micro-pipette controller of claim 17, wherein said actuator assembly and said ballonet are oriented at an end-to-end configuration while seated within said chamber of said primary housing. ### Claim 19 A method of utilizing a multi-actuated micro-pipette controller for selectively transporting a volume of fluid, said method comprising the steps of: obtaining a primary housing including a proximal end and a distal end axially opposed therefrom, a primary access port formed adjacent to said distal end, and a chamber extending from said proximal end to said distal end such that said chamber is accessible from said primary access port; obtaining and removably seating a secondary housing within said chamber, said secondary housing having a secondary access port at least partially aligned with said primary access port, wherein said secondary housing is coaxially aligned with said primary housing while said secondary housing is disposed within said chamber; obtaining a ballonet adapted to receive the fluid therein; removably inserting said ballonet within said secondary housing and at least partially aligning said ballonet with said secondary access port such that said ballonet is directly accessible and squeezable via said primary access port and said secondary access port; obtaining and locating an actuator assembly at said primary housing; operably engaging said actuator assembly with said ballonet; and causing the fluid to expel outwardly from said ballonet by linearly actuating said actuator assembly and thereby compressing at least a portion of said ballonet; wherein each of said primary access port and said second access port is spaced from said proximal end and said distal end of said primary housing.