# US 9,751,069 B2 · Compositions and methods for entrapping protein on a surface

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- Number: US 9,751,069 B2
- Document ID: US9751069B2
- Status: Granted
- Kind: B2
- Application number: 14/120,278
- Filed: 2014-05-14
- Published: 2014-11-20
- Granted: 2017-09-05
- Earliest claimed: 2013-05-14
- Assignees: Genomics USA, Inc.
- Applicants: Michael E. Hogan
- Inventors: Michael E. Hogan
- Web page: https://patentgrep.com/patent/US9751069B2

## CPC

- B01J 19/123: {Ultraviolet light}
- B01J 19/0046: {Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays (synthesis methods per se C40B50/00)}
- C07K 17/10: the carrier being a carbohydrate
- C07K 17/14: Peptides being immobilised on, or in, an inorganic carrier
- C07K 19/00: Hybrid peptides {, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes}
- C08F 2/48: by ultraviolet or visible light
- B01J 2219/00605: the compounds being directly bound or immobilised to solid supports
- B01J 2219/00612: the surface being inorganic
- B01J 2219/00626: Covalent
- B01J 2219/00637: by coating it with another layer
- B01J 2219/00659: Two-dimensional arrays
- B01J 2219/00725: Peptides
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C08F: MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS

## Abstract

The present invention provides a formulation to link protein to a solid support that comprises one or more proteins, Oligo-dT and one or more non-volatile, water-soluble protein solvents, solutes or combination thereof in an aqueous solution. Further provided is a method of attaching a protein to a surface of a substrate. The formulations provided herein are contacted onto the substrate surface, printed thereon and air dried. The substrate surface is irradiated with UV light to induce thymidine photochemical crosslinking via the thymidine moieties of the Oligo-dT.

## Claims

### Claim 1

A method of attaching a protein to a surface of a substrate, comprising the steps of:
printing one or more proteins; Oligo-dT; and one or more non-volatile, water-soluble protein solvents, solutes or combination thereof in an aqueous solution onto the substrate surface;
air-drying the substrate surface;
irradiating the substrate surface with UV light to induce thymidine photochemical crosslinking via the thymidine moieties of the Oligo-dT, thereby attaching the protein to the surface of the substrate.