# US 9,750,783 B2 · Composition containing a Spiranthes sinensis extract and pharmaceutical applications thereof

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- Number: US 9,750,783 B2
- Document ID: US9750783B2
- Status: Granted
- Kind: B2
- Application number: 14/198,932
- Filed: 2014-03-06
- Published: 2015-09-10
- Granted: 2017-09-05
- Earliest claimed: 2014-03-06
- Assignees: National Dong Hua University
- Applicants: National Dong Hua University
- Inventors: Maw-Kuen Wu; Ching-Feng Weng; Hung-Yuan Shih; Huei-Wun Sie
- Web page: https://patentgrep.com/patent/US9750783B2

## CPC

- A61K 36/898: Orchidaceae (Orchid family)
- A61P 17/18: Antioxidants, e.g. antiradicals (preparations for protection against sunlight A61Q17/00)
- A61K 9/0019: {Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner (non-active ingredients are additionally classified in A61K47/00)}
- A61K 9/08: Solutions {(composition of solutions A61K47/00)}
- A61K 2236/00: Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention is related to a pharmaceutical composition comprising an effective amount of a Spiranthes sinensis extract and a pharmaceutically acceptable carrier. The present invention improves the economic value of Spiranthes sinensis by verifying the efficacies of said pharmaceutical composition in anti-inflammation, anti-liver fibrosis and/or anti-oxidation.

## Claims

### Claim 1

A method for treating inflammation in a subject in need thereof comprising administering an effective amount of an ethyl acetate extract of Spiranthes sinensis to said subject, wherein said effective amount is 0.6 to 5 mg/60 kg body weight/day, and wherein said ethyl acetate extract of Spiranthes sinensis is obtained by:
i.) obtaining a Spiranthes sinensis plant;
ii.) mixing said Spiranthes sinensis plant with ethyl acetate to obtain a mixture, wherein ethyl acetate is the only solvent used; and
iii.) drying said mixture to obtain the ethyl extract of Spiranthes sinensis.

### Claim 2 (depends on 1)

The method of claim 1, wherein said administrating is via oral administration, intravenous injection, or a combination thereof.

### Claim 3 (depends on 1)

The method of claim 1, wherein said Spiranthes sinensis extract is administrated with a pharmaceutically acceptable carrier; selected from the group consisting of water, alcohol, glycerol, chitosan, alginate, chondroitin, Vitamin E, mineral oil, dimethyl sulfoxide (DMSO), or a combination thereof.

### Claim 4 (depends on 1)

The method of claim 1, wherein said plant is dried and/or ground before mixing with said solvent.

### Claim 5 (depends on 1)

The method of claim 1, wherein said mixing is conducted at 20 to 28° C.

### Claim 6 (depends on 1)

The method of claim 1, wherein said mixing is conducted for 1 to 2 days.

### Claim 7 (depends on 1)

The method of claim 1, wherein said drying is achieved by vacuum drying.

### Claim 8

A method for treating inflammation in a subject in need thereof comprising:
a.) preparing an ethyl acetate extract of Spiranthes sinensis by:
i.) obtaining a Spiranthes sinensis plant;
ii.) mixing said Spiranthes sinensis plant with ethyl acetate to obtain a mixture, wherein said ethyl acetate is the only solvent used; and
iii.) drying said mixture to obtain said extract; and
b.) administering 0.6 to 5 mg/60 kg body weight/day of the ethyl acetate extract of Spiranthes sinensis to the subject.