# US 9,750,773 B2 · Factors extracted from fish embryos and use of mixtures thereof in the control of stem cell multiplication and differentiation

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- Number: US 9,750,773 B2
- Document ID: US9750773B2
- Status: Granted
- Kind: B2
- Application number: 14/818,819
- Filed: 2015-08-05
- Published: 2016-02-11
- Granted: 2017-09-05
- Earliest claimed: 2014-08-07
- Applicants: Michele Biava; Maura Biava; Annamaria Ricotti
- Inventors: Piermario Biava
- Web page: https://patentgrep.com/patent/US9750773B2

## CPC

- A61K 35/60: Fish, e.g. seahorses; Fish eggs
- A61K 8/987: {of species other than mammals or birds}
- A61Q 19/08: Anti-ageing preparations
- A61K 2800/10: General cosmetic use
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS

## Abstract

A cell growth and/or differentiation factor extracted from fish embryos in selected stages of stem cell differentiation, said stages being the mid-blastula-gastrula, tail bud and pharyngula stages. The invention also provides the use of growth and differentiation factors extracted from fish embryos in the control of stem cell multiplication and differentiation.

## Claims

### Claim 1

A composition comprising of a mixture of extracts from a fish embryo in five selected stages of stem cell differentiation, said selected stages consisting of the mid-blastula-gastrula, tail bud, pharyngula, 5-somite and 20-somite stages, wherein the weight percentage of the extract from each of the five stages varies from 5% to 50% in the composition.

### Claim 2 (depends on 1)

The composition according to claim 1, wherein said fish is a zebrafish.

### Claim 3 (depends on 1)

A method for the treatment of a degenerative diseases of the human body comprising administering a therapeutically effective amount of the composition of claim 1.

### Claim 4 (depends on 3)

The method according to claim 3, wherein said degenerative disease is selected from a neurodegenerative or cardiovascular disease.

### Claim 5 (depends on 1)

A method for the treatment of cancer comprising administering a therapeutically effective amount of the composition of claim 1.

### Claim 6 (depends on 1)

A method for regenerating a human body tissue comprising administering a therapeutically effective amount of the composition according to claim 1.

### Claim 7 (depends on 1)

The composition of claim 1, further comprising a physiologically acceptable carrier.

### Claim 8 (depends on 7)

The composition according to claim 7 wherein said composition is selected from a pharmaceutical, nutraceutical or a cosmetic composition.

### Claim 9 (depends on 1)

A method for treatment of skin comprising applying a cosmetically effective amount of the composition of claim 1.

### Claim 10 (depends on 9)

The method of claim 9, comprising treatment of skin wrinkles or skin aging.