# US 9,750,699 B2 · Bioactive compounds protection method and compositions containing the same

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- Number: US 9,750,699 B2
- Document ID: US9750699B2
- Status: Granted
- Kind: B2
- Application number: 14/531,892
- Filed: 2014-11-03
- Published: 2015-04-30
- Granted: 2017-09-05
- Earliest claimed: 2003-06-20
- Assignees: Nutrinia Ltd.
- Applicants: Nutrinia Ltd.
- Inventors: Amir Barzilay; Hagit Koren-Lichtig
- Web page: https://patentgrep.com/patent/US9750699B2

## CPC

- A61K 9/19: lyophilised {, i.e. freeze-dried, solutions or dispersions (lyophilised products with subsequent particle size reduction A61K9/14; granules or pellets made by lyphilisation A61K9/1682; solid oral dosage forms made by lyophilisation A61K9/2095; lyophilisation additives A61K47/00)}
- A23K 20/147: Polymeric derivatives, e.g. peptides or proteins
- A23K 20/168: Steroids
- A23K 20/184: Hormones
- A23K 20/20: Inorganic substances, e.g. oligoelements
- A23K 40/25: by extrusion
- A23K 40/30: by encapsulating; by coating
- A23K 50/60: for weanlings
- A23L 33/10: using additives (addition of substantially indigestible substances A23L33/21)
- A61K 38/28: Insulins
- A61K 47/36: Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
- A61P 1/00: Drugs for disorders of the alimentary tract or the digestive system
- A61P 3/00: Drugs for disorders of the metabolism (of the blood or the extracellular fluid A61P7/00)
- A61P 3/10: for hyperglycaemia, e.g. antidiabetics
- A61P 11/00: Drugs for disorders of the respiratory system
- A61P 31/00: Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P 37/02: Immunomodulators
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23K: FODDER
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

The present invention relates to means for protecting bioactive materials in mammalian food or feed formulations used to enhance the health status of mammals.

## Claims

### Claim 1

A method for encapsulating or embedding a heat sensitive bioactive ingredient in a mammalian newborn food or feed formulation, comprising:
(i) mixing insulin, maltodextrin and a liquid to form a liquid blend;
(ii) drying the liquid blend of step (i) to form a dry blend;
(iii) coating the dried blend of step (ii) with an encapsulating layer comprising maltodextrin;
(iv) adding the coated dry blend of step (iii) to a mammalian newborn food or feed formulation, wherein the steps of mixing and drying are conducted at a maximum temperature of 50° C., such that the activity of the heat sensitive bioactive ingredient is substantially maintained.

### Claim 2 (depends on 1)

The method of claim 1, wherein the liquid blend of step (i) further comprises saline.

### Claim 3 (depends on 1)

The method of claim 1, wherein the insulin is recombinant human insulin.

### Claim 4 (depends on 1)

The method of claim 1, further comprising reducing the particle size of the encapsulated or embedded bioactive ingredient.

### Claim 5 (depends on 1)

The method of claim 1, wherein the method provides uniformly sized particles of an encapsulated or embedded bioactive ingredient, having an average size of between about 50 to about 1000 micrometers.

### Claim 6 (depends on 1)

The method of claim 1, wherein the mammalian newborn food or feed formulation is an infant formula, a milk replacer, or a milk substitute.

### Claim 7 (depends on 1)

The method of claim 1, wherein the mammalian newborn food or feed formulation is in the form of a powder, a solution, a suspension, an emulsion, an ointment, or a cream.

### Claim 8 (depends on 1)

The method of claim 1, wherein the maximum temperature is 37° C.

### Claim 9 (depends on 1)

A mammalian newborn food or feed formulation comprising an encapsulated or embedded heat sensitive bioactive ingredient prepared by the method of claim 1.

### Claim 10 (depends on 2)

A mammalian newborn food or feed formulation comprising an encapsulated or embedded heat sensitive bioactive ingredient prepared by the method of claim 2.

### Claim 11 (depends on 3)

A mammalian newborn food or feed formulation comprising an encapsulated or embedded heat sensitive bioactive ingredient prepared by the method of claim 3.

### Claim 12 (depends on 4)

A mammalian newborn food or feed formulation comprising an encapsulated or embedded heat sensitive bioactive ingredient prepared by the method of claim 4.

### Claim 13 (depends on 5)

A mammalian newborn food or feed formulation comprising an encapsulated or embedded heat sensitive bioactive ingredient prepared by the method of claim 5.

### Claim 14 (depends on 6)

A mammalian newborn food or feed formulation comprising an encapsulated or embedded heat sensitive bioactive ingredient prepared by the method of claim 6.

### Claim 15 (depends on 7)

A mammalian newborn food or feed formulation comprising an encapsulated or embedded heat sensitive bioactive ingredient prepared by the method of claim 7.

### Claim 16 (depends on 8)

A mammalian newborn food or feed formulation comprising an encapsulated or embedded heat sensitive bioactive ingredient prepared by the method of claim 8.