# US 9,750,687 B2 · Substance-encapsulating vesicle and process for producing the same

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- Number: US 9,750,687 B2
- Document ID: US9750687B2
- Status: Granted
- Kind: B2
- Application number: 13/699,238
- Filed: 2011-05-23
- Published: 2013-08-08
- Granted: 2017-09-05
- Earliest claimed: 2010-05-21
- Assignees: Japan Science and Technology Agency
- Applicants: Kazunori Kataoka; Akihiro Kishimura; Yasutaka Anraku; Kanjiro Miyata; Sayan Chuanoi; Tomoya Suma; Makoto Oba
- Inventors: Kazunori Kataoka; Akihiro Kishimura; Yasutaka Anraku; Kanjiro Miyata; Sayan Chuanoi; Tomoya Suma; Makoto Oba
- Web page: https://patentgrep.com/patent/US9750687B2

## CPC

- A61K 9/0087: {Galenical forms not covered by A61K9/02 - A61K9/7023}
- A61K 9/1273: Polymersomes; Liposomes with polymerisable or polymerised bilayer-forming substances
- B01J 13/02: Making microcapsules or microballoons {(for medical preparations A61K9/50)}
- B01J 13/14: Polymerisation; cross-linking
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS

## Abstract

Provided is a method for easily and efficiently producing encapsulated substance vesicles wherein a substance is encapsulated in the cavity of vesicles obtained by polymer self-assembly. Empty vesicles that have membranes comprising a first polymer that is a block copolymer with uncharged hydrophilic segments and a first kind of charged segments and a second polymer with a second kind of charged segments that carry a charge that is the opposite of said first kind of charged segments as well as spaces that are enclosed by said membranes are mixed in an aqueous medium with the substance that is to be encapsulated in the spaces.

## Claims

### Claim 1

A vesicle comprising:
a membrane containing a first polymer, which is a block copolymer having an uncharged hydrophilic segment and a first charged segment, and a second polymer, which has a second charged segment charged oppositely to the first charged segment, said membrane defining a cavity surrounded thereby and having a first charged segment to second charged segment ratio of 0.3 to less than 3.00; and
a charged substance encapsulated in the cavity.

### Claim 2 (depends on 1)

A vesicle according to claim 1, wherein the membrane has a trilaminar structure comprising an outer layer, an intermediate layer, and an inner layer.

### Claim 3 (depends on 1)

A vesicle according to claim 1, wherein the charged substance is selected from the group consisting of biomolecules, organic compounds and inorganic substances.

### Claim 4 (depends on 1)

A vesicle according to claim 1, wherein the first polymer is a block copolymer containing an uncharged hydrophilic segment and an anionic segment, and the second polymer is a block copolymer containing an uncharged hydrophilic segment and a cationic segment and/or a polymer containing a cationic segment but no uncharged hydrophilic segment.

### Claim 5 (depends on 1)

A vesicle according to claim 1, wherein the first polymer is a block copolymer containing an uncharged hydrophilic segment and a cationic segment, and the second polymer is a block copolymer containing an uncharged hydrophilic segment and an anionic segment and/or a polymer containing an anionic segment but no uncharged hydrophilic segment.

### Claim 6 (depends on 4, 5)

A vesicle according to claim 4 or 5, wherein the anionic segment is nucleic acid.

### Claim 7 (depends on 4, 5)

A vesicle according to claim 4 or 5, wherein the cationic segment is polyamine.

### Claim 8 (depends on 4, 5)

A vesicle according to claim 4 or 5, wherein the uncharged hydrophilic segment is polyalkylene glycol.