# US 9,750,682 B2 · Methods and compositions for improving the appearance of skin

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- Number: US 9,750,682 B2
- Document ID: US9750682B2
- Status: Granted
- Kind: B2
- Application number: 14/813,212
- Filed: 2015-07-30
- Published: 2017-02-02
- Granted: 2017-09-05
- Earliest claimed: 2015-07-30
- Assignees: ELC Management LLC
- Applicants: ELC Management LLC
- Inventors: Nadine Pernodet; Donald Collins
- Web page: https://patentgrep.com/patent/US9750682B2

## CPC

- A61K 8/64: Proteins; Peptides; Derivatives or degradation products thereof
- A61K 8/66: Enzymes
- A61K 8/9711: Phaeophycota or Phaeophyta [brown algae], e.g. Fucus
- A61K 8/9728: Fungi, e.g. yeasts
- A61K 8/9794: Liliopsida [monocotyledons]
- A61Q 19/08: Anti-ageing preparations
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS

## Abstract

A method for stimulating collagen synthesis in aging skin cells in need of treatment by stimulating SIRT1, SIRT3, and SIRT6 and a method for preparing a topical composition for stimulating SIRT1, SIRT3, and SIRT6 in skin cells.

## Claims

### Claim 1

A method for stimulating collagen synthesis in skin cells in need of such treatment by topically applying a composition comprising at least one extract from Laminaria genus, at least one extract from the Narcissus genus, and at least one peptide of the formula (I):
R1-(AA)n-X1-X2-X3-X4-X5-X6-(AA)p-R2, in which
X1 is glycine or threonine or histidine;
X2 is alanine or glutamine or glycine;
X3 is glycine or asparagine or serine;
X4 is valine or isoleucine or leucine;
X5 is serine or aspartic acid or phenylalanine;
X6 is alanine or glutamic acid or lysine; wherein
when X1 is glycine, then X2 is alanine and X3 is glycine;
when X1 is threonine, then X3 is asparagine;
when X1 is histidine, then X2 is glycine;
AA represents any amino acid and n and p are integers between 0 and 2;
R1 represents the primary amino function of the N-terminal amino acid, free or substituted by an acyl type group having either an alkyl chain from C1-30, saturated or unsaturated, that may be an acetyl group, or an aromatic group that may be chosen from among a benzoyl, tosyl or benzyloxycarbonyl type group; and
R2 represents the hydroxyl group of the carboxyl function of the C-terminal amino acid, free or substituted by a group that may be chosen from among an alkyl chain from C1-30, or an NH2, NHY or NYY group with Y representing an alkyl chain from C1-4 wherein the extract from the Laminaria genus is present at 0.001 to 1%, the extract from Narcissus genus is present at 0.001 to 1.5% and the peptide is present from 0.0001 to 1%, with all percentages by weight of the total composition.

### Claim 2 (depends on 1)

The method of claim 1 wherein the stimulation of collagen synthesis causes a reduction in skin laxity, a reduction in the appearance of lines or wrinkles, or both.

### Claim 3 (depends on 1)

The method of claim 1 wherein the topical composition is applied once or twice per day.

### Claim 4 (depends on 1)

The method of claim 1 wherein the topical composition is applied before a period of sustained rest.

### Claim 5 (depends on 1)

The method of claim 1 wherein the extract from the Laminaria genus stimulates SIRT3activity in skin cells.

### Claim 6 (depends on 5)

The method of claim 5 wherein the extract from the Laminaria genus is Laminaria saccharina.

### Claim 7 (depends on 1)

The method of claim 1 wherein the extract from the Narcissus genus stimulates SIRT1 activity in skin cells.

### Claim 8 (depends on 7)

The method of claim 7 wherein the extract from the Narcissus genus is Narcissus tazetta extract from the bulb in its dormant phase of growth.

### Claim 9 (depends on 1)

The method of claim 1 wherein the peptide is selected from the group consisting of:
(SEQ ID No. 1)
Glu-Ile-His-Gly-Ser-Leu-Phe-Lys-NH2
(SEQ ID No. 2)
His-Gly-Ser-Leu-Phe-Lys-NH2
(SEQ ID No. 3)
Leu-Val-Gly-Ala-Gly-Val-Ser-Ala-NH2
(SEQ ID No. 4)
Gly-Ala-Gly-Val-Ser-Ala-Glu
(SEQ ID No. 5)
Gly-Ala-Gly-Val-Ser-Ala-Glu-NH2
(SEQ ID No. 6)
Thr-Gln-Asn-Ile-Asp-Glu-Leu
(SEQ ID No. 7)
Thr-Gln-Asn-Ile-Asp-Glu-Leu-NH2,
and
(SEQ ID No. 8)
Val-Ile-Thr-Gln-Asn-Ile-Asp-Ala-NH2.

### Claim 10 (depends on 9)

The method of claim 9 wherein the peptide is of the following formula:
(SEQ ID No. 5)
Gly-Ala-Gly-Val-Ser-Ala-Glu-NH2.

### Claim 11 (depends on 1)

The method of claim 1 wherein the peptide incorporated into the composition is in the form of yeast extract with the peptide being a component of the extract.

### Claim 12 (depends on 1)

The method of claim 1 wherein the extract from the Laminaria genus is Laminaria digitata.

### Claim 13 (depends on 1)

The method of claim 1 wherein the extract from the Laminaria genus is one or more of Laminaria digitata or Laminaria saccharina and the extract from Narcissus is Narcissus tazetta.

### Claim 14 (depends on 1)

The method of claim 1 wherein the skin cells are dermal fibroblasts.