# US 9,750,675 B2 · Use of green light to activate L-amino acid oxidase

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- Number: US 9,750,675 B2
- Document ID: US9750675B2
- Status: Granted
- Kind: B2
- Application number: 12/668,689
- Filed: 2008-07-16
- Published: 2010-09-09
- Granted: 2017-09-05
- Earliest claimed: 2007-07-16
- Assignees: L'oreal
- Applicants: Julien Laboureau; Quang Lan Nguyen
- Inventors: Julien Laboureau; Quang Lan Nguyen
- Web page: https://patentgrep.com/patent/US9750675B2

## CPC

- A61K 8/44: Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
- A61K 8/442: {substituted by amido group(s)}
- A61K 8/447: {containing sulfur}
- A61K 8/4953: {containing pyrimidine ring derivatives, e.g. minoxidil}
- A61K 8/4973: {with oxygen as the only hetero atom}
- A61K 8/673: {Vitamin B group}
- A61N 5/0616: {Skin treatment other than tanning}
- A61Q 19/08: Anti-ageing preparations
- A61K 2800/78: Enzyme modulators, e.g. Enzyme agonists
- A61K 2800/81: Preparation or application process involves irradiation
- A61N 5/0617: {Hair treatment}
- A61N 5/067: using laser light
- A61N 2005/0652: {Arrays of diodes}
- A61N 2005/0654: {Lamps}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61N: ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY (measurement of bioelectric currents A61B; surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body A61B18/00; anaesthetic apparatus in general A61M; incandescent lamps H01K; infrared radiators for heating H05B)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS

## Abstract

The present invention relates to the use of a source of green light for activating L-amino acid oxidase in the presence of at least one substrate of this enzyme in order to stimulate the energy metabolism of the cells of the skin, to stimulate their replacement and thus to improve the appearance of the skin and hair, in particular to prevent and/or treat cutaneous signs of ageing of the skin and hair.

## Claims

### Claim 1

A method, comprising:
contacting a surface of skin of a subject with a composition consisting of one or more of ammonium polyacryldimethyltauramide, cyclohexasiloxane, glycerol, stearyl alcohol, glyceryl stearate/PEG-100 stearate, dimyristyl tartrate/cetearyl alcohol/C12-15 pareth-7/PPG-25 laureth 25, xanthan gum, a preservative, and water; and at least one substrate selected from the group consisting of leucine, serine, threonine, glycine, alanine, valine, phenylalanine, tyrosine, lysine, arginine, cysteine, aspartate, glutamate, citrulline, ornithine, diaminopimelic acid, penicillamine and α-aminobutyric acid, wherein the skin comprises L-amino acid oxidase EC 1.4.3.2; and
activating the L-amino acid oxidase in the presence of the at least one substrate by exposing the surface of skin with green light having a wavelength of from 500 to 580 nm,
wherein the exposure to green light is obtained from a source selected from the group consisting of a laser, an IPL, and a metabolite or an active principle that emits light between 500 and 580 nm on exposure to UV radiation.

### Claim 2 (depends on 1)

The method according to claim 1, wherein a concentration of the at least one substrate in the composition is from 0.001 to 10 weight percent.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the composition is a topical composition.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the source is a metabolite or active principle that emit light between 500 and 580 nm on exposure to UV radiation and is selected from the group consisting of vitamin B2, baicailine in acid medium, fisetin, dipyridamole, a pigment and a mineral.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the source of green light provides light power in a range of from 500 to 90,000 mJ/cm2.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the substrate is alanine, leucine or a combination thereof.

### Claim 7 (depends on 6)

The method according to claim 6, wherein the substrate is leucine.

### Claim 8 (depends on 6)

The method according to claim 6, wherein the substrate is alanine.