# US 9,750,445 B2 · Porous polymeric formulation prepared using porogens

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- Number: US 9,750,445 B2
- Document ID: US9750445B2
- Status: Granted
- Kind: B2
- Application number: 13/930,877
- Filed: 2013-06-28
- Published: 2015-01-01
- Granted: 2017-09-05
- Earliest claimed: 2013-06-28
- Assignees: Verily Life Sciences LLC
- Applicants: Verily Life Sciences LLC
- Inventors: Zenghe Liu; Jeffrey George Linhardt; Huanfen Yao
- Web page: https://patentgrep.com/patent/US9750445B2

## CPC

- A61B 5/1486: using enzyme electrodes, e.g. with immobilised oxidase
- A61B 5/14532: {for measuring glucose, e.g. by tissue impedance measurement}
- A61B 5/14507: {specially adapted for measuring characteristics of body fluids other than blood (devices for taking samples of body liquids A61B10/0045)}
- A61B 5/14546: {for measuring analytes not otherwise provided for, e.g. ions, cytochromes}
- A61B 5/682: {Mouth, e.g., oral cavity; tongue; Lips; Teeth}
- A61B 5/6821: {Eye}
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)

## Abstract

An analyte sensor for the continuous or semi-continuous monitoring of physiological parameters and a method for making the analyte sensor are disclosed. The analyte sensor includes a crosslinked copolymer network in contact with a surface of an electrode. The copolymer network has voids formed by the removal of a porogen, and an analyte sensing component is immobilized within the network. The method involves forming a solution of the precursors of the copolymer, depositing the mixture on a surface of an electrode, and curing the deposited mixture to provide the analyte sensor.

## Claims

### Claim 1

An analyte sensor comprising:
a crosslinked copolymer network in contact with a surface of an electrode, wherein the crosslinked copolymer network consists of:
backbone chains comprising first methacrylate-derived units and second methacrylate-derived units, wherein each first methacrylate-derived unit has a side chain; and
crosslinks between the second methacrylate-derived units in different backbone chains, wherein the crosslinks comprise poly(alkylene oxide);
an analyte sensing component embedded within the crosslinked copolymer network; and
voids within and defined by the crosslinked copolymer network, wherein the voids are large enough to allow an analyte to pass through the crosslinked copolymer network and small enough to not allow the analyte sensing component to pass through the crosslinked copolymer network.

### Claim 2 (depends on 1)

The sensor according to claim 1, wherein the side chain of the first methacrylate-derived units comprise one or more hydroxy groups.

### Claim 3 (depends on 1)

The sensor according to claim 1, wherein the first methacrylate-derived units have the structure of formula (I):
wherein
X is —O—, —NR′— or —S—;
y is 0-10; and
R1 is hydrogen, —C1-C12alkyl, —C1-C12alkyl-OH, —SiR′3, —C(O)—C1-C12alkyl, —C1-C12alkyl-C(O)OR′, wherein R′ is —C1-C12alkyl.

### Claim 4 (depends on 1)

The sensor according to claim 1, wherein the first methacrylate-derived units have the structure:

### Claim 5 (depends on 1)

The sensor according to claim 1, wherein the crosslinks have the structure of formula (IIa):
wherein w is 2-10.

### Claim 6 (depends on 1)

The sensor according to claim 1, wherein the crosslinks have the structure of formula (IIa):
wherein w is an average value of from about 2 to about 250.

### Claim 7 (depends on 1)

The sensor according to claim 1, wherein the crosslinks are derived from the di(ethylene glycol) portion of di(ethylene glycol) dimethacrylate.

### Claim 8 (depends on 1)

The sensor according to claim 1, wherein the analyte sensing component comprises glucose oxidase.

### Claim 9 (depends on 1)

The sensor according to claim 1, wherein the crosslinked copolymer network has a thickness of about 10 μm to about 30 μm.

### Claim 10 (depends on 1)

The sensor according to claim 1, wherein the first methacrylate-derived units have the structure of formula (I):
wherein
X is —O—, —NR′— or —S—;
y is 0-10; and
R1 is hydrogen, —C1-C12alkyl, —C1-C12alkyl-OH, —SiR′3, —C(O)—C1-C12alkyl, —C1-C12alkyl-C(O)OR′, wherein R′ is —C1-C12alkyl;
the crosslinks have the structure of formula (IIa):
wherein w is 2-10; and
the analyte sensing component comprises glucose oxidase.

### Claim 11 (depends on 8)

The sensor of claim 8, wherein the analyte is glucose and the voids within and defined by the crosslinked copolymer network are large enough to allow glucose to pass through the crosslinked copolymer network and small enough to not allow glucose oxidase to pass through the crosslinked copolymer network.