# US 9,750,244 B2 · Antimicrobially active compositions

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- Number: US 9,750,244 B2
- Document ID: US9750244B2
- Status: Granted
- Kind: B2
- Application number: 14/531,796
- Filed: 2014-11-03
- Published: 2015-03-05
- Granted: 2017-09-05
- Earliest claimed: 2005-04-07
- Assignees: Xy, LLC; CHD Bioscience, Inc.
- Applicants: Xy, LLC; CHD Bioscience, Inc.
- Inventors: Edwin Dean Neas; Jerald Edward Kuiken; John Louis Schenk; Thomas Boyd Gilligan
- Web page: https://patentgrep.com/patent/US9750244B2

## CPC

- A01N 31/02: Acyclic compounds
- A01N 25/00: Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application {, e.g. seed treatment or sequential application}; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N 31/06: Oxygen or sulfur directly attached to a cycloaliphatic ring system
- A01N 35/02: containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
- A01N 35/06: containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
- A61L 2/18: Liquid substances
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)

## Abstract

Compositions (3) and methods of using such compositions (3) to condition, clean, or disinfect the flow path of a conduit (8) of a microfluidic devices (16), such as flow cytometers or liquid chromatographs.

## Claims

### Claim 1

An antimicrobial composition, comprising:
an alcohol about 10% to about 55% by volume selected from the group consisting of methanol, ethanol, N-propanol, isopropanol, 2-butanol, n-butanol and isobutanol;
a ketone about 2% to about 10% by volume selected from the group consisting of acetone, 2-butanone, cyclohexanone, 2-pentanone, 3-pentanone, 2-hexanone, 3-hexanone, methyl isobutyl ketone, cyclobutanone, and cyclopentanone, said alcohol and said ketone being the only effective antimicrobially active components in said antimicrobial composition; and
an inactive agent comprising water, wherein said alcohol and said ketone remain miscible in said water, and wherein said alcohol and said ketone combined in amounts which kill viable microorganisms by greater than 2-log10 within a time period of about five minutes to about thirty minutes.

### Claim 2 (depends on 1)

The antimicrobial composition of claim 1, wherein said alcohol comprises about 30% to about 50% by volume, and wherein said ketone comprises about 2% to about 10% by volume.

### Claim 3 (depends on 1)

The antimicrobial composition of claim 1, wherein said composition is selected from the group consisting of: methanol 50%, acetone 10%, and water 40% v/v; methanol 50%, 2-butanone 10%, and water 40% v/v; methanol 50%, cyclohexanone 10%, and water 40% v/v; ethanol 50%, acetone 10%, and water 40% v/v; ethanol 50%, 2-butanone 10%, and water 40% v/v; ethanol 50%, cyclohexanone 10%, and water 40% v/v; isopropanol 50%, acetone 10%, and water 40% v/v; isopropanol 50%, 2-butanone 10%, and water 40% v/v; isopropanol 50%, cyclohexanone 10%, and water 40% v/v; N-propanol 50%, acetone 10%, and water 40% v/v; N-propanol 50%, 2-butanone 10%, and water 40% v/v; N-propanol 50%, cyclohexanone 10%, and water 40% v/v; 2-butanol 50%, acetone 10%, and water 40% v/v; 2-butanol 50%, 2-butanone 10%, and water 40% v/v; and 2-butanol 50%, cyclohexanone 10%, and water 40% v/v.

### Claim 4 (depends on 1)

The antimicrobial composition of claim 1, wherein said alcohol and said ketone comprise isopropanol 50% and acetone 10% by volume.

### Claim 5 (depends on 1)

The antimicrobial composition of claim 1, wherein said viable microorganisms occur within a flow path of a microfluidic instrument, said antimicrobial composition has a flow within said flow path of said microfluidic instrument to kill said viable microorganisms within said flow path by greater than 2-log10 within said time period of about five minutes to about thirty minutes.

### Claim 6 (depends on 5)

The antimicrobial composition of claim 5, wherein said flow of said antimicrobial composition in said flow path of said microfluidic device generated by an amount of pressure exerted on the external surface of a variable volume container containing said antimicrobial composition.

### Claim 7 (depends on 6)

The antimicrobial composition of claim 6, wherein said flow of said antimicrobial composition in said flow path of a microfluidic device occurs in a flow cytometer.