# US 9,750,222 B2 · Spinach hybrid SV3319VC and parents thereof

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- Number: US 9,750,222 B2
- Document ID: US9750222B2
- Status: Granted
- Kind: B2
- Application number: 14/498,443
- Filed: 2014-09-26
- Published: 2016-01-14
- Granted: 2017-09-05
- Earliest claimed: 2014-07-14
- Assignees: Seminis Vegetable Seeds, Inc.
- Applicants: Seminis Vegetable Seeds, Inc.
- Inventors: Bernardus Baerends
- Web page: https://patentgrep.com/patent/US9750222B2

## CPC

- A01H 6/028: {Spinacia oleracea [spinach]}
- A01H 5/12: Leaves
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

The invention provides seed and plants of spinach hybrid SV3319VC and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of spinach hybrid SV3319VC and the parent lines thereof, and to methods for producing a spinach plant produced by crossing such plants with themselves or with another spinach plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the leaf and gametes of such plants.

## Claims

### Claim 1

A spinach plant comprising at least a first set of the chromosomes of spinach line SSBS012-1191F, a sample of seed of said line having been deposited under ATCC Accession Number PTA-121441.

### Claim 2

A seed comprising at least a first set of the chromosomes of spinach line SSBS012-1191F, a sample of seed of said line having been deposited under ATCC Accession Number PTA-121441.

### Claim 3 (depends on 1)

The plant of claim 1, which is an inbred.

### Claim 4 (depends on 1)

The plant of claim 1, which is a hybrid.

### Claim 5 (depends on 2)

The seed of claim 2, which is an inbred.

### Claim 6 (depends on 2)

The seed of claim 2, which is a hybrid.

### Claim 7 (depends on 4)

The plant of claim 4, wherein the hybrid plant is spinach hybrid SV3319VC, a sample of seed of said hybrid SV3319VC having been deposited under ATCC Accession Number PTA-121453.

### Claim 8 (depends on 6)

The seed of claim 6, wherein the seed is a seed of spinach hybrid SV3319VC, a sample of seed of said hybrid SV3319VC having been deposited under ATCC Accession Number PTA-121453.

### Claim 9 (depends on 1)

A plant part of the plant of claim 1.

### Claim 10 (depends on 9)

The plant part of claim 9, wherein the plant part is a fruit, an ovule, pollen, a leaf, or a cell.

### Claim 11 (depends on 7)

A spinach plant having all the physiological and morphological characteristics of the spinach plant of claim 7.

### Claim 12 (depends on 1)

A tissue culture of regenerable cells of the plant of claim 1.

### Claim 13 (depends on 12)

The tissue culture according to claim 12, comprising cells or protoplasts from a plant part selected from the group consisting of embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, pistil, flower, seed and stalks.

### Claim 14 (depends on 12)

A spinach plant regenerated from the tissue culture of claim 12, wherein said plant comprises all of the morphological and physiological characteristics of the spinach plant comprising at least a first set of the chromosomes of spinach line SSBS012-1191F, a sample of seed of said line having been deposited under ATCC Accession Number PTA-121441.

### Claim 15 (depends on 1)

A method of vegetatively propagating the spinach plant of claim 1 comprising the steps of:
(a) collecting tissue capable of being propagated from said spinach plant;
(b) cultivating said tissue to obtain proliferated shoots; and
(c) rooting said proliferated shoots to obtain a rooted plantlet.

### Claim 16 (depends on 15)

The method of claim 15, further comprising growing at least a first plant from said rooted plantlet.

### Claim 17

A method of introducing one desired trait into spinach line SSBS012-1191F, said method comprising:
(a) crossing a plant of line SSBS012-1191F with a second spinach plant that comprises the desired trait to produce F1 progeny, a sample of seed of said line having been deposited under ATCC Accession Number PTA-121441;
(b) selecting an F1 progeny that comprises the desired trait;
(c) backcrossing the selected F1 progeny with a plant of the line SSBS012-1191F to produce backcross progeny;
(d) selecting a backcross progeny comprising the desired trait; and
(e) repeating steps (c) and (d) three or more times to produce a selected fourth or higher backcross progeny that comprises the desired trait, and otherwise comprises all of the morphological and physiological characteristics of the line SSBS012-1191F.

### Claim 18 (depends on 17)

A spinach plant produced by the method of claim 17.

### Claim 19

A method of producing a spinach plant comprising an added trait, the method comprising introducing a transgene conferring the trait into a plant of hybrid SV3319VC, or line SSBS012-1191F, a sample of seed of said hybrid and line having been deposited under ATCC Accession Number PTA-121453, and ATCC Accession Number PTA-121441, respectively.

### Claim 20 (depends on 19)

A spinach plant produced by the method of claim 19.

### Claim 21 (depends on 20)

The plant of claim 20, wherein the transgene confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

### Claim 22 (depends on 1)

The plant of claim 1, further comprising a single locus conversion.

### Claim 23 (depends on 22)

The plant of claim 22, wherein the single locus conversion confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

### Claim 24

A method for producing a seed of a spinach plant derived from at least one of hybrid SV3319VC, or line SSBS012-1191F comprising the steps of:
(a) crossing a spinach plant of hybrid SV3319VC, or line SSBS012-1191F with itself or a second spinach plant; a sample of seed of said hybrid and line having been deposited under ATCC Accession Number PTA-121453, and ATCC Accession Number PTA-121441, respectively; and
(b) allowing a seed of the hybrid SV3319VC-derived, or line SSBS012-1191F-derived spinach plant to form.

### Claim 25 (depends on 24)

The method of claim 24, further comprising the steps of:
(c) selfing a plant grown from said hybrid SV3319VC-derived, or SSBS012-1191F-derived spinach seed to yield an additional hybrid SV3319VC-derived, or line SSBS012-1191F-derived spinach seed;
(d) growing said additional hybrid SV3319VC-derived, or line SSBS012-1191F-derived spinach seed of step (c) to yield an additional hybrid SV3319VC-derived, or line SSBS012-1191F-derived spinach plant; and
(e) repeating the crossing and growing steps of (c) and (d) to generate at least a first further hybrid SV3319VC, or line SSBS012-1191F-derived spinach plant.

### Claim 26 (depends on 24)

The method of claim 24, wherein the second spinach plant is of an inbred spinach line.

### Claim 27 (depends on 25)

The method of claim 25, further comprising:
(f) crossing the further hybrid SV3319VC-derived, or SSBS012-1191F-derived spinach plant with a second spinach plant to produce a seed of a hybrid progeny plant.

### Claim 28 (depends on 7)

A plant part of the plant of claim 7.

### Claim 29 (depends on 28)

The plant part of claim 28, wherein the plant part is a fruit, an ovule, pollen, a leaf, or a cell.

### Claim 30 (depends on 1)

A method of producing a spinach seed comprising crossing the plant of claim 1 with itself or a second spinach plant and allowing seed to form.

### Claim 31 (depends on 1)

A method of producing a spinach fruit comprising:
(a) obtaining the plant according to claim 1, wherein the plant has been cultivated to maturity; and
(b) collecting a spinach fruit from the plant.