# US 9,750,216 B2 · Hybrid sunflower X4237

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- Number: US 9,750,216 B2
- Document ID: US9750216B2
- Status: Granted
- Kind: B2
- Application number: 15/092,857
- Filed: 2016-04-07
- Published: 2016-10-13
- Granted: 2017-09-05
- Earliest claimed: 2015-04-10
- Assignees: Nuseed Americas Inc.
- Applicants: Nuseed Americas Inc.
- Inventors: Scott Arthur Radi; Erin Marie Gerdes
- Web page: https://patentgrep.com/patent/US9750216B2

## CPC

- A01H 6/1464: {Helianthus annuus [sunflower]}
- A01H 5/02: Flowers
- A01H 5/10: Seeds
- C12N 15/8241: {Phenotypically and genetically modified plants via recombinant DNA technology}
- C12N 15/8274: {for herbicide resistance}
- C12N 15/8275: {Glyphosate}
- C12N 15/8277: {Phosphinotricin}
- C12N 15/8278: {Sulfonylurea}
- C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance}
- C12N 15/8281: {for bacterial resistance}
- C12N 15/8282: {for fungal resistance}
- C12N 15/8283: {for virus resistance}
- C12N 15/8286: {for insect resistance}
- C12N 15/8289: {Male sterility}
- C12N 5/14: Plant cells
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A hybrid sunflower, designated X4237, is disclosed. The invention relates to the seeds of hybrid sunflower X4237, to the plants of hybrid sunflower X4237 and to methods for producing a sunflower plant by crossing the cultivar X4237 with itself or another sunflower. The invention further relates to methods for producing a sunflower plant containing in its genetic material one or more transgenes and to the transgenic sunflower plants and plant parts produced by those methods and to methods for producing other hybrid sunflower derived from hybrid sunflower X4237.

## Claims

### Claim 1

A hybrid sunflower plant designated X4237, wherein a representative sample of seed of said hybrid sunflower was deposited under ATCC Accession No. PTA-123220.

### Claim 2 (depends on 1)

A hybrid sunflower plant having all of the physiological and morphological characteristics of the sunflower plant of claim 1.

### Claim 3 (depends on 1)

A tissue culture produced from protoplasts or cells from the plant of claim 1, wherein said cells or protoplasts are produced from a plant part selected from the group consisting of leaf, pollen, embryo, cotyledon, hypocotyl, meristematic cell root, root tip, pistil, anther, ovule, flower, shoot, stem, and petiole.

### Claim 4 (depends on 3)

A sunflower plant regenerated from the tissue culture of claim 3, wherein the plant has all of the morphological and physiological characteristics of hybrid sunflower X4237.

### Claim 5 (depends on 1)

A method for producing a sunflower seed, said method comprising crossing two sunflower plants and harvesting the resultant sunflower seed, wherein at least one sunflower plant is the sunflower plant of claim 1.

### Claim 6 (depends on 5)

The method of claim 5, wherein one of said sunflower plants is hybrid sunflower X4237 and the other is transgenic.

### Claim 7 (depends on 1)

A method of producing a male sterile sunflower plant, wherein the method comprises introducing a nucleic acid molecule that confers male sterility into the sunflower plant of claim 1.

### Claim 8 (depends on 7)

A male sterile sunflower plant produced by the method of claim 7.

### Claim 9 (depends on 1)

A method of producing an herbicide resistant sunflower plant, wherein said method comprises introducing a gene conferring herbicide resistance into the plant of claim 1, wherein the gene is selected from the group consisting of glyphosate, sulfonylurea, imidazolinone, dicamba, glufosinate, phenoxy proprionic acid, L-phosphinothricin, cyclohexone, cyclohexanedione, triazine, and benzonitrile.

### Claim 10 (depends on 9)

An herbicide resistant sunflower plant produced by the method of claim 9.

### Claim 11 (depends on 1)

A method of producing a pest or insect resistant sunflower plant, wherein said method comprises introducing a gene conferring pest or insect resistance into the plant of claim 1.

### Claim 12 (depends on 11)

A pest or insect resistant sunflower plant produced by the method of claim 11.

### Claim 13 (depends on 12)

The sunflower plant of claim 12, wherein the gene encodes a Bacillus thuringiensis endotoxin.

### Claim 14 (depends on 1)

A method of producing a disease resistant sunflower plant, wherein said method comprises introducing a gene conferring disease resistance into the plant of claim 1.

### Claim 15 (depends on 14)

A disease resistant sunflower plant produced by the method of claim 14.

### Claim 16 (depends on 1)

A method of producing a sunflower plant wherein said method comprises introducing a transgene into the plant of claim 1.

### Claim 17 (depends on 16)

A sunflower plant produced by the method of claim 16.