# US 9,750,208 B1 · Maize hybrid X85F778

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- Number: US 9,750,208 B1
- Document ID: US9750208B1
- Status: Granted
- Kind: B1
- Application number: 14/623,591
- Filed: 2015-02-17
- Granted: 2017-09-05
- Earliest claimed: 2014-03-06
- Assignees: Pioneer Hi-Bred International, Inc.
- Applicants: Pioneer Hi Bred International Inc
- Inventors: Gerhard P Weber; Lori Karyn Scott; Jean-Marc Montpetit
- Web page: https://patentgrep.com/patent/US9750208B1

## CPC

- A01H 6/4684: {Zea mays [maize]}
- A01H 5/10: Seeds
- C12N 15/8243: {involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine}
- C12N 15/8245: {involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis}
- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C12N 15/8251: {Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis}
- C12N 15/8271: {for stress resistance, e.g. heavy metal resistance}
- C12N 15/8274: {for herbicide resistance}
- C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance}
- C12N 15/8286: {for insect resistance}
- C12N 15/8289: {Male sterility}
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A novel maize variety designated X85F778 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X85F778 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X85F778 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X85F778, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X85F778. This invention further relates to methods for producing maize varieties derived from maize variety X85F778.

## Claims

### Claim 1

An F1 hybrid maize variety X85F778 seed, representative F1 hybrid seed produced by crossing a first plant of variety PH1W4Z with a second plant of variety PH1MJ9, wherein representative seed of said varieties PH1W4Z and PH1MJ9 have been deposited under ATCC Accession Numbers PTA-122441 and PTA-123178, respectively.

### Claim 2 (depends on 1)

The hybrid maize variety X85F778 seed of claim 1, further comprising a seed treatment.

### Claim 3 (depends on 1)

A method comprising cleaning the hybrid maize variety X85F778 seed of claim 1.

### Claim 4 (depends on 1)

A method comprising generating a molecular marker profile from nucleic acids isolated from the hybrid maize variety X85F778 seed of claim 1.

### Claim 5 (depends on 1)

A plant, plant part, or plant cell produced by growing the hybrid maize variety X85F778 seed of claim 1, the plant, plant part or plant cell comprising at least one F1 hybrid maize variety X85F778 cell.

### Claim 6 (depends on 5)

A method comprising generating a molecular marker profile from nucleic acids isolated from the plant, plant part, or plant cell of claim 5.

### Claim 7 (depends on 5)

A method of producing a commodity plant product comprising starch, syrup, silage, fat or protein, the method comprising producing the commodity plant product from the plant or plant part of claim 5.

### Claim 8 (depends on 5)

A method for producing a second maize plant, the method comprising applying plant breeding techniques to the maize plant or plant part of claim 5 to produce the second maize plant.

### Claim 9 (depends on 5)

A method comprising:
(a) crossing the maize plant or plant part of claim 5 with itself or a different plant to produce progeny seed;
(b) growing the progeny seed to produce a progeny plant and crossing the progeny plant with itself or a different plant to produce further progeny seed; and
(c) repeating step (b) for at least an additional two generations to produce a second maize plant.

### Claim 10 (depends on 5)

A method for producing a second maize plant, the method comprising doubling haploid seed generated from a cross of the maize plant or plant part of claim 5 with a different maize plant.

### Claim 11

An F1 hybrid maize variety X85F778 seed further comprising a locus conversion, the seed further comprising a locus conversion being produced by crossing a first plant of variety PH1W4Z with a second plant of variety PH1MJ9; representative seed of said varieties PH1W4Z and PH1MJ9 having been deposited under ATCC Accession Numbers PTA-122441 and PTA-123178, respectively; wherein at least one of said varieties PH1W4Z and PH1MJ9 further comprises a locus conversion and wherein the seed further comprising a locus conversion produces a plant having otherwise the same morphological and physiological characteristics as maize variety X85F778 when grown under the same environmental conditions.

### Claim 12 (depends on 11)

The seed further comprising a locus conversion of claim 11, further comprising a seed treatment.

### Claim 13 (depends on 11)

A method comprising cleaning the seed further comprising a locus conversion of claim 11.

### Claim 14 (depends on 11)

The seed further comprising a locus conversion of claim 11, wherein the locus conversion confers a property selected from the group consisting of male sterility, site-specific recombination, abiotic stress tolerance, altered phosphorus, altered antioxidants, altered fatty acids, altered essential amino acids, altered carbohydrates, herbicide tolerance, insect resistance and disease resistance.

### Claim 15 (depends on 11)

A method comprising generating a molecular marker profile from nucleic acids isolated from the hybrid maize variety X85F778 seed of claim 11.

### Claim 16 (depends on 11)

A plant, plant part, or plant cell produced by growing the hybrid maize variety X85F778 seed of claim 11, the plant, plant part or plant cell comprising at least one F1 hybrid maize variety X85F778 cell.

### Claim 17 (depends on 16)

A method comprising generating a molecular marker profile from nucleic acids isolated from the plant, plant part, or plant cell of claim 16.

### Claim 18 (depends on 16)

A method of producing a commodity plant product comprising starch, syrup, silage, fat or protein, the method comprising producing the commodity plant product from the plant or plant part of claim 16.

### Claim 19 (depends on 16)

A method for producing a second maize plant, the method comprising applying plant breeding techniques to the plant or plant part of claim 16 to produce the second maize plant.

### Claim 20 (depends on 16)

A method for producing a second maize plant, the method comprising doubling haploid seed generated from a cross of the maize plant or plant part of claim 16 with a different maize plant.