# US 9,750,206 B2 · HO/LL canola with resistance to clubroot disease

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- Number: US 9,750,206 B2
- Document ID: US9750206B2
- Status: Granted
- Kind: B2
- Application number: 13/842,535
- Filed: 2013-03-15
- Published: 2013-11-07
- Granted: 2017-09-05
- Earliest claimed: 2011-02-09
- Assignees: Dow AgroSciences LLC
- Applicants: Dow AgroSciences LLC
- Inventors: Gregory R. Gingera; Jianwei Zhao; Van Leonard Ripley; Lasantha Ubayasena
- Web page: https://patentgrep.com/patent/US9750206B2

## CPC

- A01H 1/00: Processes for modifying genotypes {; Plants characterised by associated natural traits} (A01H4/00 takes precedence)
- A01H 1/045: {using molecular markers}
- A01H 5/10: Seeds
- A01H 6/202: {Brassica napus [canola]}
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- C12Q 2600/158: Expression markers
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

This disclosure concerns a plant of the genus, Brassica, or parts thereof, which comprise one or more traits selected from the group consisting of high oleic acid content, low linolenic acid content, increased herbicide resistance, restorer of cytoplasmic male sterility, and increased clubroot disease (Plasmodiophora brassicae) resistance, compared to a wild-type plant of the same species. This disclosure further relates to wild-type and mutant alleles of genes involved in these traits, molecular markers linked thereto, and methods of their use.

## Claims

### Claim 1

A method for identifying a clubroot resistant plant comprising a mutation in a fad3 gene contributing to a low linolenic acid phenotype in Brassica napus, the method comprising:
isolating genomic DNA comprising a clubroot resistance gene from a plant;
determining the presence in the isolated genomic DNA of a polynucleotide that is amplified in a polymerase chain reaction by the primers of SEQ ID NO:18 and SEQ ID NO:19 and is 300 nucleotides in length; and
determining the presence in the isolated genomic DNA of a polynucleotide that is specifically hybridizable with the complement of SEQ ID NO:12, wherein the polynucleotide comprises a single nucleotide polymorphism (SNP) nucleic acid molecular marker that is an adenine at the position corresponding to the first base of a 5′ splice site in the third intron of the Brassica napus fad32 gene contributing to a low linolenic acid phenotype in Brassica napus.

### Claim 2 (depends on 1)

The method according to claim 1, wherein determining the presence in the isolated genomic DNA of the polynucleotide that is specifically hybridizable with the complement of SEQ ID NO:12 comprises contacting the isolated genomic DNA with an oligonucleotide comprising the nucleotide sequence of SEQ ID NO:15.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the polynucleotide that is specifically hybridizable with the complement of SEQ ID NO:12 is not hybridizable with the complement of SEQ ID NO:13.