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US 9,745,573 B2Granted
Compositions and methods for preparing oligonucleotide solutions
AssigneeIllumina, Inc.
InventorsJohn R. Stuelpnagel, Mark Chee
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Abstract
The present invention is directed to methods and compositions for generating a pool of oligonucleotides. The invention finds use in preparing a population or subpopulations of oligonucleotides in solution. The pool of oligonucleotides finds use in a variety of nucleic acid detection and/or amplification assays.
Claims16
A method of detecting target nucleic acids comprising:
- (i) generating a pool of oligonucleotides comprising:
- (a) providing a first substrate and at least 50 different single-stranded oligonucleotides linked to said first substrate through cleavable linkers;
- and
- (b) cleaving said linkers, thereby releasing said single-stranded oligonucleotides from said first substrate as a pool of oligonucleotides comprising said oligonucleotides;
- (ii) hybridizing the target nucleic acids with the pool of oligonucleotides;
- (iii) capturing the hybridized pool of single-stranded oligonucleotides on a second substrate;
- and
- (iv) detecting the target nucleic acids.
5 more elements
A method of detecting target nucleic acids comprising:
- (i) generating a pool of oligonucleotides comprising:
- (a) obtaining a first substrate comprising a plurality of single-stranded oligonucleotides linked to said first substrate, wherein the plurality of single-stranded oligonucleotides are different from one another, wherein the plurality of single-stranded oligonucleotides linked to said first substrate comprises at least 50 oligonucleotides,
- (b) releasing the plurality of single-stranded oligonucleotides from the first substrate as a pool of single-stranded oligonucleotides;
- (ii) hybridizing the pool of single-stranded oligonucleotides with the target nucleic acids;
- (iii) capturing the hybridized pool of oligonucleotides on a second substrate;
- and
- (iv) detecting the target nucleic acids.
4 more elements
Dependencies are inferred from the claim text.