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US 9,745,554 B2Granted
Method of improving cell proliferation of pancreatic progenitor cells in a pancreatic cell culture
AssigneeReneuron Limited
InventorsMonica Florio, Aleksandar Francki, Wen-Ghih Tsang
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Abstract
The invention relates to the discovery that the proliferation and survival of pancreatic progenitor cells can be enhanced by contacting the cells with, (1) a caspase inhibitor sufficient to reduce apoptosis in the pancreatic endocrine cells; and, (2) a growth factor in an amount sufficient to increase the level of activated Akt in the pancreatic endocrine cells.
Claims10
A method of improving insulin or glucagon production in mature pancreatic endocrine cells in a pancreatic cell culture comprising
- contacting pancreatic endocrine cells with (1) an exogenous caspase inhibitor in an amount sufficient to reduce apoptosis in the pancreatic endocrine cells;
- and (2) at least one exogenous growth factor in an amount sufficient to increase the level of activated Akt in the pancreatic endocrine cells;
- and
- differentiating the pancreatic endocrine cells contacted with (1) and (2) into mature pancreatic endocrine cells that express insulin and/or glucagon at a synergistically higher level than differentiated pancreatic endocrine cells contacted only with (1) or (2), wherein glucagon and/or insulin are as measured by qPCR analysis of insulin mRNA or glucagon mRNA, respectively, and wherein the qPCR results are as displayed as the ratio of insulin mRNA to β-actin mRNA, or glucagon mRNA to actin mRNA, respectively.
1 more element
Dependencies are inferred from the claim text.