Method for generating a parvovirus B19 virus-like particle
AssigneeNorvartis AG
InventorsEthan Settembre, Angelica Medina-Selby, Doris Coit, Philip R. Dormitzer
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Abstract
The invention provides a process for generating parvovirus VP1/VP2 virus like particles (VLPs). The invention further provides methods for purification of the parvovirus VLPs and immunogenic compositions that contain the VLPs. The invention also includes recombinant nucleic acid molecules that encode parvovirus VP1 and VP2, and host cells that contain the recombinant nucleic acids.
Claims8
A recombinant nucleic acid comprising a nucleotide sequence encoding parvoviris VP1 protein (VP1) operably linked to a first control element and a nucleotide sequence encoding parvovirus VP2 protein (VP2) operably linked to a second control element, wherein the recombinant nucleic acid comprises equivalent amounts of the nucleotide sequence encoding VP1 and the nucleotide sequence encoding VP2, the first control element is a weak promoter suitable for expression in a yeast cell and the second control element is a strong promoter suitable for expression in a yeast cell, wherein the second control element is an ADH/GAPDH promoter comprising SEQ ID NO:
- 19 and the first control element is a variant of said ADH/GAPDH promoter that results in decreased expression of VP1 relative VP2, wherein, when recombinant nucleic acid is expressed in a yeast cell, VP1 is produced in a lower abundance relative to VP2, and virus like particles (VLP) are formed that comprise VP1 and VP2.
Dependencies are inferred from the claim text.