Methods, reagents and cells for biosynthesizing compounds
AssigneeInvista North America S.A.R.L.
InventorsAlex Van Eck Conradie, Adriana Leonora Botes, Ramdane Haddouche
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Abstract
This document describes biochemical pathways for producing 7-hydroxyheptanoate methyl ester and heptanoic acid heptyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 7-hydroxyheptanoate methyl esters and heptanoic acid heptyl esters can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol.
Claims11
A method of producing one or more terminal hydroxy-substituted (C4-9 alkyl)-OC(═O)—(C3-8 alkyl) esters in a recombinant host, said method comprising:
- a) enzymatically converting a C4-9 alkanoyl-CoA to a (C4-9 alkyl)-OC(═O)—(C3-8 alkyl) ester in said recombinant host, wherein the C4-9 alkanoyl-CoA is enzymatically converted to the (C4-9 alkyl)-OC(═O)—(C3-8 alkyl) ester using a polypeptide having alcohol O-acetyltransferase activity, wherein said polypeptide having alcohol O-acetyltransferase activity is classified under EC 2.3.1.-;
- and
- b) enzymatically converting the (C4-9 alkyl)-OC(═O)—(C3-8 alkyl) ester to a (C4-9 alkyl)-OC(═O)—(C3-8 hydroxyalkyl) ester, (C4-9 hydroxyalkyl)-OC(═O)—(C3-8 hydroxyalkyl) ester, or (C4-9hydroxyalkyl)-OC(═O)—(C3-8alkyl) ester using a polypeptide having monooxygenase activity in said recombinant host, wherein said polypeptide having monooxygenase activity is classified under EC 1.14.14.- or EC 1.14.15.-,
- the method optionally further comprising enzymatically converting the (C4-9 hydroxyalkyl)-OC(═O)—(C3-8 hydroxyalkyl) ester or the (C4-9 alkyl)-OC(═O)—(C3-8 hydroxyalkyl) ester to a C4-9 hydroxyalkanoate using a polypeptide having esterase activity classified under EC 3.1.1.-.
Dependencies are inferred from the claim text.