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US 9,737,620 B2Granted
Vectors and methods for genetic immunization
AssigneeNature Technology Corporation
InventorJames A. Williams
Life of this patent
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Abstract
Improved DNA vaccine plasmids are disclosed that contain novel immunostimulatory RNA compositions. The improved plasmids eliminate all extraneous sequences, incorporate a novel antibiotic free short RNA based selectable marker, increase eukaryotic expression using a novel chimeric promoter, improve yield and stability during bacterial production, and improve immunostimulation. These vectors are utilized in immunization to elicit improved immune responses or therapy to induce type 1 interferon production.
Claims6
A composition of matter for a antibiotic marker-free covalently closed, super-coiled DNA replicon comprising:
- a. an RNA-OUT antisense RNA gene sequence comprising an internal insertion sequence 10 derived portion comprising a RNA-OUT promoter sequence and a RNA-OUT sequence portion that is complementary to an RNA-IN gene sequence and a terminator sequence wherein said RNA-OUT antisense RNA gene sequence has at least 90% sequence identity to the sequence set forth as SEQ ID NO:
- 15, operably linked to,
- b. an antibiotic marker-free covalently closed, super-coiled DNA replicon comprising a gene of interest.
Dependencies are inferred from the claim text.