Isolation, cultivation and uses of stem/progenitor cells
AssigneeCellresearch Corporation Pte. Ltd.
InventorsToan-Thang Phan, Ivor Jiun Lim
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method of cultivating stem/progenitor cells of the amniotic membrane of umbilical cord that comprises obtaining a tissue explant from the amniotic membrane of umbilical cord and cultivating the tissue explant in suitable cultivation media and cultivation conditions over a suitable period of time. The method may include isolating the stem/progenitor cells from the tissue cultures. The stem cells may be epithelial or mesenchymal stem/progenitor cells. The isolated stem cell cells can have embryonic stem cell-like properties and can be used for various therapeutic purposes.
Claims4
A method of cultivating a mesenchymal stem/progenitor cell population of the amniotic membrane of umbilical cord, the mesenchymal stem/progenitor cell population having the capacity to differentiate in multiple cell types, the method comprising:
- obtaining a tissue explant from the amniotic membrane of umbilical cord;
- cultivating the tissue explant under conditions allowing cell proliferation of the mesenchymal stem/progenitor cells without differentiation of the mesenchymal stem/progenitor cells;
- and
- isolating the stem/progenitor cells of the amniotic membrane, wherein the stem/progenitor cells have a spindle shape, express the following genes:
- POU5f1, Bmi-1, leukemia inhibitory factor (LIF), and wherein the stem/progenitor cells secrete Activin A and follistatin.
Dependencies are inferred from the claim text.