Sharply resolving labeled protein molecular weight standards
AssigneeLife Technologies Corporation
InventorsRoumen Bogoev, Douglas Kang
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Abstract
Pre-labeled protein standards useful in electrophoresis that have sharp, consistent separation characteristics that are substantially the same as those of their unlabeled counterparts are provided. The invention provides pre-labeled protein standard sets that include a plurality of labeled proteins that are labeled on a first amino acid, in which side reactions of the label with amino acids not targeted for labeling are reduced.
Claims4
A pre-labeled protein standard set comprising a plurality of labeled proteins, each protein having a known and different molecular weight ranging from 10 Da to 260 kDa, wherein one or more of said plurality of labeled proteins is a selectively labeled protein comprising a heterologous chromophore or a heterologous fluorophore label conjugated to a first amino acid, wherein said selectively labeled protein has fewer than one lysine residue per ten kilodalton (kDa) molecular weight of said selectively labeled protein, wherein at least a first selectively labeled protein comprises an amino acid sequence that has at least 80% sequence identity with the amino acid sequence of SEQ ID NO:
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Dependencies are inferred from the claim text.