US 9,732,365 B2Granted

Biosynthesis of paclitaxel intermediate

AssigneeBoard of Trustees of Michigan State University

InventorKevin Walker

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The invention relates to methods of making compounds useful for production of paclitaxel and analogs or derivatives thereof.

Dependent claims

A method comprising:

reacting a substrate represented by formula I with a tyrocidine synthetase (TycA) to produce an aminopropanoyl-CoA of formula II,
wherein:
X is hydrogen;
Y is hydrogen or OH;
and Ring is an unsubstituted or substituted aryl, heteroaryl, (C4-C10) cycloalkyl, or (C4-C9) heterocycloakyl;
mixing said aminopropanoyl-CoA of formula II with Baccatin III of the formula:
to form a mixture;
and
reacting said mixture with a baccatin III O-phenylpropanoyltransferase (BAPT) to produce N-debenzoyl-2′-deoxypaclitaxel of the formula:
wherein each of X, Y, and Ring has the same meaning as defined above.

7 more elements

Dependencies are inferred from the claim text.

C12P 17/02
Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms: oxygen as only ring hetero atoms
C12N 9/90
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: isomerases
C12Y 501/01011
Acting on amino acids and derivatives: phenylalanine racemase
C12P
Fermentation or enzyme-using processes to synthesise a desired chemical compound or composition

From the US filing history. Foreign family members aren’t listed.

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