Stacked herbicide tolerance event 8264.42.32.1, related transgenic soybean lines, and detection thereof

A soybean seed comprising a genome comprising Event pDAB8264.42.32.1 as present in representative seed deposited with American Type Culture Collection (ATCC) under Accession No. PTA-11993.

A method of making an expression cassette for a soybean plant, said method comprising producing a heterologous polynucleotide, operably linked to a promoter, and inserting said heterologous polynucleotide operably linked to said promoter into a segment of soybean genomic DNA to produce SEQ ID NO:18.

An isolated polynucleotide molecule, wherein said molecule has a sequence having SEQ ID NO:18.

An isolated polynucleotide, wherein said polynucleotide comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs:18, 19, 20, and 21.

A method of breeding a soybean plant, said method comprising crossing a first soybean plant comprising a transgenic insert in genomic DNA of said first soybean plant, said transgenic insert having a sequence having SEQ ID NO:18, with a second soybean plant to produce a third soybean plant, and assaying said third soybean plant for the presence of said transgenic insert.

A stably transformed dicot plant comprising a polynucleotide having a nucleic acid molecule comprising a sequence selected from the group consisting of SEQ ID NO:18, SEQ ID NO:19, and SEQ ID NO:20.

A plant cell comprising a polynucleotide segment having SEQ ID NO:18 in its genome.

An isolated polynucleotide having a sequence selected from the group consisting of SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, and the complements thereof.