WNT7A compositions and method of using the same
AssigneeOttawa Hospital Research Institute
InventorsMichael A. Rudnicki, Conrad Florian Bentzinger, Radoslav Zinoviev
Life of this patent
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Abstract
There are provided compositions and methods for modulating stem cell division, in particular, division symmetry. It has been demonstrated that Wnt7a polypeptide fragments promoting symmetrical expansion of stem cells. The compositions and methods of the invention are useful, for example, in modulating stem cell division symmetry in vitro, ex vivo, and in vivo, in replenishing and expanding the stem cell pool, and in promoting the formation, maintenance, repair and regeneration of tissue.
Claims26
A composition for increasing the symmetric expansion of stem cells comprising (a) a Wnt7a polypeptide fragment having the amino acid sequence set forth in SEQ ID NO:
- 5, (b) a Wnt7a polypeptide fragment having an N-terminal deletion of 210 to 219 amino acids in the amino acid sequence set forth in SEQ ID NO:
- 3, or (c) a polynucleotide encoding the polypeptide of (a) or (b).
A method for increasing the symmetric expansion of stem cells comprising contacting the stem cells with a composition comprising (a) a Wnt7a polypeptide fragment having the amino acid sequence set forth in SEQ ID NO:
- 5, (b) a Wnt7a polypeptide fragment having an N-terminal deletion of 210 to 219 amino acids in the amino acid sequence set forth in SEQ ID NO:
- 3, or (c) a polynucleotide encoding the polypeptide of (a) or (b).
A method for promoting muscle formation, regeneration, maintenance or repair in a subject comprising administering to the subject a composition comprising (a) a Wnt7a polypeptide fragment having the amino acid sequence set forth in SEQ ID NO:
- 5, (b) a Wnt7a polypeptide fragment having an N-terminal deletion of 210 to 219 amino acids in the amino acid sequence set forth in SEQ ID NO:
- 3, or (c) a polynucleotide encoding (a) or (b).
Dependencies are inferred from the claim text.