Spatially addressable molecular barcoding
AssigneeCellular Research, Inc.
InventorsStephen P. A. Fodor, Christina Fan, Glenn Fu, Geoffrey Facer
Life of this patent
Estimating the term…
Abstract
The disclosure provides for methods, compositions, systems, devices, and kits for determining the number of distinct targets in distinct spatial locations within a sample. In some examples, the methods include: stochastically barcoding the plurality of targets in the sample using a plurality of stochastic barcodes, wherein each of the plurality of stochastic barcodes comprises a spatial label and a molecular label; estimating the number of each of the plurality of targets using the molecular label; and identifying the spatial location of each of the plurality of targets using the spatial label. The method can be multiplexed.
Claims30
A method for determining spatial locations of a plurality of single cells, comprising:
- stochastically barcoding the plurality of singe cells using a plurality of synthetic particles,
- wherein each of the plurality of synthetic particles comprises a plurality of stochastic barcodes, a first group of optical labels, and a second group of optical labels,
- wherein each of the plurality of stochastic barcodes comprises a cellular label and a molecular label,
- wherein each optical label in the first group of optical labels comprises a first optical moiety and each optical label in the second group of optical labels comprises a second optical moiety, and
- wherein each of the plurality of synthetic particles is associated with an optical barcode comprising the first optical moiety and the second optical moiety;
- detecting the optical barcode of each of the plurality of synthetic particles to determine the location of each of the plurality of synthetic particles;
- and
- determining the spatial locations of the plurality of single cells based on the locations of the plurality of synthetic particles.
A synthetic particle, comprising:
- a plurality of stochastic barcodes, wherein each of the plurality of stochastic barcodes comprises a cellular label and a molecular label;
- a first group of optical labels;
- and
- a second group of optical labels,
- wherein each optical label in the first group of optical labels comprises a first optical moiety and each optical label in the second group of optical labels comprises a second optical moiety, and
- wherein each of the plurality of synthetic particles is associated with an optical barcode comprising the first optical moiety and the second optical moiety.
Dependencies are inferred from the claim text.