Chromatographic medium

A chromatographic medium comprising:

• a base layer;
• a first layer comprising a permeation layer for enabling the permeation of at least one target substance to be separated by a separating agent layer and disposed in a first plane, the permeation layer containing a porous material and a fluorescent indicator or coloring reagent as constituent materials;
• and
• a second layer comprising a separating agent layer for separating the at least one target substance and disposed in a second plane different from the first plane,
• wherein the separating agent layer is laminated to the base layer,
• the at least one target substance, the separating agent layer and the permeation layer all exhibit an optical responsiveness to ultraviolet rays, the permeation layer exhibits an optical responsiveness to ultraviolet rays different from the at least one target substance and the separating agent layer and the permeation layer is laminated to a region of a surface of the separating agent layer by applying a slurry containing the porous material and the fluorescent indicator or coloring reagent and then drying the slurry and not laminated to another region of the surface of the separating agent layer.