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US 9,725,739 B2Granted
Method for preparing D-chiro-inositol using microbes
AssigneeDy Natural Co., Ltd.,
InventorsHyun Koon Myeong, Sang-Hwal Yoon, Hyeon-Seo Lee
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Abstract
The present invention relates to a method for preparing D-chiro-inositol from myo-inositol by using a transformed host cell which expresses enzymes such as a myo-inositol transporter, inositol dehydrogenase, and inosose isomerase. According to the method of the present invention, myo-inositol can be converted into D-chiro-inositol at a high yield.
Claims6
A method for preparing D-chiro-inositol from myo-inositol, comprising:
- (a) transforming a host cell with (i) a recombinant vector comprising a myo-inositol transporter coding DNA sequence operatively linked to a promoter;
- and (ii) a recombinant vector comprising an inositol dehydrogenase coding DNA sequence and an inosose isomerase coding DNA sequence operatively linked to a promoter;
- and
- (b) culturing the host cell of (a) in a medium comprising myo-inositol, thereby preparing D-chiro-inositol,
- wherein the host cell is selected from the group consisting of a mammalian cell, an insect cell, a yeast cell, and a prokaryotic cell,
- wherein the myo-inositol transporter has the amino acid sequence set forth in SEQ ID NO:
- 3,
- wherein the inositol dehydrogenase has the amino acid sequence set forth in SEQ ID NO:
- 31, and
- wherein the inosose isomerase has the amino acid sequence set forth in SEQ ID NO:
- 30.
8 more elements
A host cell, transformed with a recombinant vector, the recombinant vector comprising:
- (i) a myo-inositol transporter coding DNA sequence operatively linked to a promoter;
- and (ii) an inositol dehydrogenase coding DNA sequence and an inosose isomerase coding DNA sequence which are operatively linked to a promoter,
- wherein the host cell is selected from the group consisting of a mammalian cell, an insect cell, a yeast cell, and a prokaryotic cell,
- wherein the myo-inositol transporter has the amino acid sequence set forth in SEQ ID NO:
- 3,
- wherein the inositol dehydrogenase has the amino acid sequence set forth in SEQ ID NO:
- 31, and
- wherein the inosose isomerase has the amino acid sequence set forth in SEQ ID NO:
- 30.
6 more elements
A recombinant vector kit for preparing D-chiro-inositol from myo-inositol, comprising:
- (i) a recombinant vector comprising a myo-inositol transporter DNA coding sequence operatively linked to a promoter;
- and (ii) a recombinant vector comprising an inositol dehydrogenase coding DNA sequence and an inosose isomerase coding DNA sequence operatively linked to a promoter as an active component,
- wherein the myo-inositol transporter has the amino acid sequence set forth in SEQ ID NO:
- 3,
- wherein the inositol dehydrogenase has the amino acid sequence set forth in SEQ ID NO:
- 31, and
- wherein the inosose isomerase has the amino acid sequence set forth in SEQ ID NO:
- 30.
5 more elements
Dependencies are inferred from the claim text.