Mutant gibberellin 2-oxidase genes and uses thereof
AssigneeAcademia Sinica
InventorsSu-May Yu, Shuen-Fang Lo, Liang-Jwu Chen, Tuan-Hua David Ho
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Abstract
The present invention relates to mutant gibberellin 2-oxidase (GA2ox) genes and uses thereof. In particular, the effective mutations disclosed herein can reduce GA2ox enzymatic activity to different extents, leading to various degrees of GA deficient yet beneficial agronomic traits in transgenic plants.
Claims18
An isolated polynucleotide that encodes a mutant class C20 gibberellin 2-oxidase protein (C20 GA2ox), wherein the mutant C20 GA2ox protein includes an amino acid mutation selected from the group consisting of:
- (i) an amino acid residue corresponding to position 123 of SEQ ID NO:
- 1 substituted with Alanine (123A),
- (ii) an amino acid residue corresponding to position 140 of SEQ ID NO:
- 1 substituted with Alanine (140A),
- (iii) an amino acid residue corresponding to position 141 of SEQ ID NO:
- 1 substituted with Glutamate (141E),
- (iv) an amino acid residue corresponding to position 143 of SEQ ID NO:
- 1 substituted with Alanine (143A), and
- (v) an amino acid residue corresponding to position 343 of SEQ ID NO:
- 1 substituted with Alanine (343A).
An expression vector comprising a nucleotide sequence that encodes a mutant class C20 gibberellin 2-oxidase protein (C20 GA2ox), wherein the mutant C20 GA2ox protein includes an amino acid mutation selected from the group consisting of:
- (i) an amino acid residue corresponding to position 123 of SEQ ID NO:
- 1 substituted with Alanine (123A),
- (ii) an amino acid residue corresponding to position 140 of SEQ ID NO:
- 1 substituted with Alanine (140A),
- (iii) an amino acid residue corresponding to position 141 of SEQ ID NO:
- 1 substituted with Glutamate (141E),
- (iv) an amino acid residue corresponding to position 143 of SEQ ID NO:
- 1 substituted with Alanine (143A), and
- (v) an amino acid residue corresponding to position 343 of SEQ ID NO:
- 1 substituted with Alanine (343A).
A transgenic plant comprising a transgene, wherein the transgene encodes a mutant class C20 gibberellin 2-oxidase protein (C20 GA2ox), wherein the mutant C20 GA2ox protein includes an amino acid mutation selected from the group consisting of:
- (i) an amino acid residue corresponding to position 123 of SEQ ID NO:
- 1 substituted with Alanine (123A),
- (ii) an amino acid residue corresponding to position 140 of SEQ ID NO:
- 1 substituted with Alanine (140A),
- (iii) an amino acid residue corresponding to position 141 of SEQ ID NO:
- 1 substituted with Glutamate (141E),
- (iv) an amino acid residue corresponding to position 143 of SEQ ID NO:
- 1 substituted with Alanine (143A), and
- (v) an amino acid residue corresponding to position 343 of SEQ ID NO:
- 1 substituted with Alanine (343A).
Dependencies are inferred from the claim text.