Kit for preparation of target radiopharmaceuticals and method of using it

A method for preparing target radiopharmaceuticals, which comprises the steps of:

• (1) mixing a vial B and a vial A and reacting the same at an appropriate temperature for an appropriate period, wherein the vial A contains lyophilized mixture of N,N-bis(2-mercaptoethyl)-N′,N′-diethyl-ethylenediamine(BMEDA), sodium gluconate, and stannous chloride, and the vial B contains an aqueous solution of radionuclide including 188Re and/or 186Re lyophilized, and wherein the lyophilized mixture is prepared by the steps of:
• a) mixing BMEDA, sodium gluconate, and stannous chloride to produce a mixture;
• b) freezing the mixture at −80° C. and flushing the same with N2 gas under 0.120 mBar for 1 hour;
• c) lyophilizing the mixture at −80° C. and flushing the same with N2 gas under 0.120 mBar for 36 hours;
• and
• c) lyophilizing the mixture at 18° C. and flushing the same with N2 gas under the pressure of 0.120 mBar for 12 hours;
• (2) adjusting the pH of the mixture obtained in step (1) in a range of 6˜7 by adding aqueous sodium hydroxide solution, and
• (3) injecting the liposome solution in a vial C into the mixture obtained in step (2) and reacting at an appropriate temperature for an appropriate period to obtain a target pharmaceutical, i.e., 188Re-and/or 186Re-Liposome, wherein the vial C contains an aqueous liposome solution comprising phospholipid, cholesterol, and 1,2-distearoyl-sn-glycero-3- phosphoethanolamine-N-[maleimide (polyethylene glycol)-2000] (DSPE-PEG 2000) in a mole ratio of 30˜95:20˜45:3˜7.5.