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US 9,715,099 B2Granted
Maskless imaging of dense samples using multi-height lensfree microscope
AssigneeThe Regents of the University of California
InventorsAydogan Ozcan, Alon Greenbaum
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Abstract
A method of imaging includes illuminating a sample spaced apart from an image sensor at a multiple distances. Image frames of the sample obtained at each distance are registered to one another and lost phase information from the registered higher resolution image frames is iteratively recovered. Amplitude and/or phase images of the sample are reconstructed based at least in part on the recovered lost phase information.
Claims10
A method of imaging a sample comprising:
- illuminating a sample spaced apart from an image sensor at a first distance with an illumination source;
- obtaining a hologram image of the sample from an image sensor with the sample spaced apart from the image sensor at the first distance;
- illuminating the sample spaced apart from an image sensor at a one or more distances different from the first distance with an illumination source;
- obtaining a hologram image of the sample from an image sensor with the sample spaced apart from the image sensor at the one or more distances different from the first distance;
- registering the hologram images of the sample obtained at the one or more distances and the hologram image of the sample obtained at the first distance;
- recovering lost phase information from the registered images;
- and
- reconstructing an amplitude or phase image of the sample based at least in part on the recovered lost phase information, wherein the hologram images of the sample are obtained without any lenses positioned between the illumination source and the image sensor.
5 more elements
A method of imaging a sample comprising:
- illuminating a sample spaced apart from an image sensor at a first distance with an illumination source at a plurality of different locations;
- obtaining lower resolution image frames of the sample from an image sensor at plurality of different locations with the sample spaced apart from the image sensor at the first distance;
- recovering a higher resolution image of the sample at the first distance based at least in part on the plurality of lower resolution image frames obtained at the first distance;
- illuminating the sample spaced apart from an image sensor at a one or more distances different from the first distance with an illumination source at a plurality of different locations;
- obtaining lower resolution image frames of the sample from an image sensor at plurality of different locations with the sample spaced apart from the image sensor at the one or more distances different from the first distance;
- recovering a higher resolution image of the sample at the one or more distances based at least in part on the plurality of lower resolution image frames obtained at the one or more distances;
- registering the higher resolution images of the sample obtained at the one or more distances and the higher resolution image of the sample obtained at the first distance;
- iteratively recovering lost phase information from the registered higher resolution images;
- and
- reconstructing an amplitude or phase image of the sample based at least in part on the recovered lost phase information.
7 more elements
Dependencies are inferred from the claim text.