Method for producing patchoulol and 7-epi-α-selinene
AssigneeFirmenich SA
InventorsMichel Schalk, Fabienne Deguerry
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Abstract
The present invention provides a method of producing patchoulol and 7-epi-α-selinene, said method comprising contacting at least one polypeptide with famesyl phyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce patchoulol and 7-epi-α-seline, compounds which can be useful in the field of perfumery. The present invention also provides the amino acid sequence of polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing patchoul and 7-epi-α-selinene is also an object of the present invention.
Claims7
A method for producing a mixture of terpenes comprising one or more of patchoulol and 7-epi-α-selinene comprising
- a) contacting FPP with at least one polypeptide having sesquiterpene synthase activity and comprising an amino acid sequence at least 90% identical to SEQ ID NO:1 to produce a mixture of terpenes;
- b) optionally, isolating the mixture of terpenes comprising one or more of patchoulol and 7-epi-α-selinene produced in step a).
Dependencies are inferred from the claim text.