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US 9,714,439 B2Granted
Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system
AssigneeCodexis, Inc.
InventorsFabien Louis Cabirol, Steven J. Collier, Thomas Daussmann, Naga K. Modukuru
Life of this patent
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Abstract
The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.
Claims24
A process for converting a compound mixture of formula IId which comprises a substrate for an amino acid dehydrogenase to a composition of formula I and a chiral amino acid of formula IIa:
- comprising contacting the compound mixture of formula IId with an enantioselective amino acid L-leucine dehydrogenase in a reaction medium comprising NAD+/NADH or NADP+/NADPH and a cofactor recycling system comprising a ketoreductase comprising the amino acid sequence of SEQ ID NO:
- 18, and a lower alkyl ketone, under conditions where the compound mixture of formula IId is converted to the composition of formula I and a chiral amino acid of formula IIa, and the lower alkyl ketone is converted to a lower secondary alcohol;
- wherein R is a substituted or unsubstitued —(C1-C10)alkyl, —(C2-C6)alkenyl, —(C2-C6)alkynl, —(C3-C8)cycloalkyl, heterocycloalky, aryl, or heteroaryl.
Dependencies are inferred from the claim text.