Modified santalene synthase polypeptides, encoding nucleic acid molecules and uses thereof
AssigneeEvolva SA
InventorsJean Davin Amick, Bryan N. Julien
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Abstract
Provided are modified santalene synthase polypeptides, nucleic acid molecules encoding the modified santalene synthase polypeptides, and methods of using the modified santalene synthase polypeptides. The modified santalene synthase polypeptides include those that catalyze production of increased levels of terpenes or altered profiles thereof or both.
Claims25
A nucleic acid molecule encoding a modified santalene synthase polypeptide comprising the sequence of amino acids set forth in any of SEQ ID NOS:
- 244 or 309-311, or a nucleic acid molecule encoding a synthase that has at least 95% sequence identity to the sequence set forth in any of SEQ ID NOS:
- 244 or 309-311, wherein
- the encoded modified santalene synthase polypeptide:
- i) catalyzes the production of total terpene product(s) from farnesyl diphosphate (FPP) in a host cell in an amount that is greater than the amount of the same total terpene products produced from FPP by the santalene synthase encoded by the sequence of nucleotides set forth in SEQ ID NO:3 in the same host cell and under the same conditions;
- or
- ii) catalyzes the production of terpene product(s) from farnesyl diphosphate (FPP) in a host cell with an altered product profile compared to the profile of the terpene products produced from FPP by the santalene synthase encoded by the sequence of nucleotides set forth in SEQ ID NO:3 in the same host cell and under the same conditions;
- the host cell is a cell that produces FPP;
- and
- the terpene product or products is a sesquiterpene.
A nucleic acid molecule encoding a modified santalene synthase polypeptide comprising the sequence of amino acids set forth in any of SEQ ID NOS:
- 244 or 309-311, or a nucleic acid molecule encoding a synthase that has at least 95% sequence identity to the sequence set forth in any of SEQ ID NOS:
- 244 or 309-311, wherein:
- the modified santalene synthase catalyzes the production of total terpene product(s) from farnesyl diphosphate (FPP) in a host cell in an amount that is greater than the amount of the same total terpene products produced from FPP by the santalene synthase encoded by the sequence of nucleotides set forth in SEQ ID NO:3 in the same host cell and under the same conditions, wherein:
- the host cell is a cell that produces FPP;
- and
- the terpene product or products is a sesquiterpene.
A nucleic acid molecule encoding a modified santalene synthase polypeptide comprising the sequence of amino acids set forth in any of SEQ ID NOS:
- 244 or 309-311, or a nucleic acid molecule encoding a synthase that has at least 95% sequence identity to the sequence set forth in any of SEQ ID NOS:
- 244 or 309-311, wherein
- the modified santalene synthase catalyzes the production of terpene products from farnesyl diphosphate (FPP) in a host cell with an altered product profile compared to the profile of the terpene products produced from FPP by the santalene synthase encoded by the sequence of nucleotides set forth in SEQ ID NO:3 in the same host cell and under the same conditions, wherein:
- the host cell is a cell that produces FPP;
- and
- the terpene product or products is a sesquiterpene.
Dependencies are inferred from the claim text.