Biosensors and biosensing incorporating RF and microwave radiation
AssigneeIntel Corporation
InventorMoran Horesh
Life of this patent
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Abstract
Devices capable applying microwave and RF electromagnetic radiation to specific molecular interactions between a capture molecule and a target analyte and capable of sensing the specific molecular interaction are provided. Specific molecular interactions include, for example, specific recognition events such as, receptor-ligand, antigen-antibody, DNA-protein, sugar-lectin, RNA-ribosome, and enzyme substrate interactions and nucleic acid-nucleic acid hybridizations. Additionally, methods are provided for detecting the presence or absence of a target analyte in a sample. The presence or absence of the target analyte is detected, in part, through the detection of a binding complex between the target analyte and a capture molecule in the presence of microwave and or RF electromagnetic radiation.
Claims22
A device capable of sensing a biological interaction, wherein the device comprises
- a substrate having a surface and a field effect transistor wherein the field effect transistor has a sensing surface and the sensing surface is in the substrate surface and wherein the field effect transistor is capable of sensing the occurrence of biological events at the sensing surface and wherein the biological events are an occurrence of an enzymatic catalytic reaction,
- a first module capable of generating electromagnetic radiation having a frequency in the microwave or radiofrequency range housed in the substrate, wherein the first module comprises a transmitting element connected to a circuit to cause the production of microwave or radiofrequency radiation by the transmitting element, and
- a second module for causing the first module capable of generating electromagnetic radiation to irradiate the sensing surface with a known frequency of microwave or radiofrequency electromagnetic radiation wherein the known frequency of microwave or radiofrequency electromagnetic radiation is selected to enhance a signal obtained by the field effect transistor for a molecule to be detected relative to molecules that are not to be detected;
- and
- an enzyme on the sensing surface capable of catalyzing a chemical reaction in the presence of a target analyte.
A device capable of sensing a biological interaction, wherein the device comprises
- a substrate having a surface and a sensor wherein the sensor has a sensing element that is comprised of an electrode having a surface and the electrode is in the substrate surface and wherein the sensor is capable of sensing the occurrence of biological events at the electrode surface and wherein the electrode surface comprises capture molecules capable of specifically recognizing a target analyte,
- a first module capable of generating electromagnetic radiation having a frequency in the microwave or radiofrequency range housed in the substrate, wherein the first module comprises a transmitting element connected to a circuit to cause the production of microwave or radiofrequency radiation by the transmitting element, and
- a second module for causing the first module capable of generating electromagnetic radiation to irradiate the electrode surface with a known frequency of microwave or radiofrequency electromagnetic radiation wherein the known frequency of microwave or radiofrequency electromagnetic radiation is selected to enhance a signal obtained by the sensing element for a molecule to be detected relative to molecules that are not to be detected;
- and
- an enzyme on the electrode surface capable of catalyzing a chemical reaction in the presence of the target analyte.
A device capable of sensing a biological interaction, wherein the device comprises:
- a substrate;
- a field effect transistor (FET), which includes a sensing surface, on the substrate;
- wherein:
- (a)(i) the FET is capable of sensing the occurrence of biological events at the sensing surface, and (a)(ii) the biological events are an occurrence of an enzymatic catalytic reaction;
- a first module on the substrate and including at least one transmitting element;
- wherein the first module and the at least one transmitting element are configured to scan electromagnetic radiation over a frequency range included within a range of 3 Hz-300 GHz;
- a second module to cause the first module to scan the radiation over the frequency range and onto the sensing surface;
- wherein the frequency range is configured to enhance a signal obtained by the FET for a molecule to be detected relative to molecules that are not to be detected, and
- an enzyme on the sensing surface capable of catalyzing a chemical reaction in the presence of a target analyte.
Dependencies are inferred from the claim text.