Method and device for in vivo desorption ionization of biological tissue
AssigneeSemmelweis Egyetem
InventorZoltan Takats
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Abstract
The invention relates to method and apparatus for production of gaseous ions from components of a condensed phase sample and analysis thereof, wherein one or more liquid jet(s) is/are directed to the surface of the sample to be investigated, where the impact of the liquid jet on the sample surface produces droplets carrying sample particles which are turned into gaseous ions via the evaporation of liquid or, if desired, by a subsequent ionization after the evaporation and the obtained sample particles are analyzed by a known method.
Claims24
A method of analyzing a sample, the method comprising:
- applying a laser beam to the sample to both cut into the sample a depth of greater than 10 μm and simultaneously convert at least a portion of the sample into gaseous sample particles, wherein the gaseous sample particles comprise gaseous ions and sample particles convertible into gaseous ions;
- transporting at least some of the gaseous sample particles to an ion analyzer unit;
- and
- analyzing at least some of the gaseous sample particles with the ion analyzer unit.
A system for analyzing a sample, the system comprising:
- a device configured to apply a laser beam to both cut into the sample a depth of greater than 10 μm and simultaneously convert at least a portion of the sample into gaseous sample particles, wherein the gaseous sample particles comprise gaseous ions and sample particles convertible into gaseous ions;
- a sample collector unit configured to transport the gaseous sample particles from the sample to an ion analyzer;
- and
- an ion analyzer operationally coupled to the sample collector unit, the ion analyzer configured to generate data for identifying components of the sample.
A method for analyzing a sample, the method comprising:
- applying a laser beam to cut into the sample a depth of greater than 10 μm, wherein the laser cuts by forming a cavity on the surface of the sample with an increasing depth as a function of time;
- dislocating ions and sample particles convertible into ions from the sample using the laser beam;
- converting at least a portion of the sample into gaseous ions by other than solvent action;
- and
- analyzing the obtained gaseous ions.
Dependencies are inferred from the claim text.