Method for producing lower alkyl ester
AssigneeAjinomoto Co., Inc.
InventorsRustem Saidovich Shakulov, Elena Vitalievna Klyachko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh
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Abstract
A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.
Claims12
A method for producing a lower alkyl ester of α-L-aspartyl-L-phenylalanine, comprising:
- A) cultivating a bacterium of Escherichia coli in a culture medium which is able to produce and accumulate L-phenylalanine in the medium, and
- B) synthesizing the lower alkyl ester of α-L-aspartyl-L-phenylalanine from aspartic acid or a derivative thereof and the L-phenylalanine obtained in step A);
- wherein the bacterium comprises a DNA comprising:
- i) a gene selected from the group consisting of tyrA, pheA, pgi, ilvE, ilvA, tdcB, sdaA, sdaB, argA, argG, proB, thrB, and combinations thereof, and
- ii) a DNA fragment able to be transcribed and encoding the peptide of SEQ ID NO:
- 2, or a variant thereof consisting of a deletion, insertion, substitution, or addition of 1 amino acid as compared to SEQ ID NO:
- 2;
- and
- wherein said DNA fragment of ii) is attached to the 3′ end of said gene of i) and consequently enhances production of an L-amino acid.
Dependencies are inferred from the claim text.