Cells and methods for producing fatty alcohols
AssigneeWisconsin Alumni Research Foundation
InventorsBrian F. Pfleger, J. Tyler Youngquist
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Abstract
Recombinant cells and methods for improved yield of fatty alcohols. The recombinant cells harbor a recombinant thioesterase gene, a recombinant acyl-CoA synthetase gene, and a recombinant acyl-CoA reductase gene. In addition, a gene product from one or more of an acyl-CoA dehydrogenase gene, an enoyl-CoA hydratase gene, a 3-hydroxyacyl-CoA dehydrogenase gene, and a 3-ketoacyl-CoA thiolase gene in the recombinant cells is functionally deleted. Culturing the recombinant cells produces fatty alcohols at high yields.
Claims17
A recombinant cell for producing a fatty alcohol comprising a recombinant thioesterase gene, a recombinant acyl-CoA synthetase gene, and a recombinant acyl-CoA reductase gene, wherein the acyl-CoA reductase gene is configured to be present in the cell in exponential phase at a copy number of from 1 to 5 copies per copy of genomic DNA, wherein a gene in the cell selected from the group consisting of an acyl-CoA dehydrogenase gene, an enoyl-CoA hydratase gene, a 3-hydroxyacyl-CoA dehydrogenase gene, and a 3-ketoacyl-CoA thiolase gene is deleted, and wherein the recombinant cell is capable of producing a fatty alcohol.
Dependencies are inferred from the claim text.