Oleaginous bacterial cells and methods for producing lipids
AssigneeNeste Oyj
InventorsStephan Hetzler, Stefan Uthoff, Daniel Broker, Alexander Steinbuchel, Perttu Koskinen
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Abstract
This invention relates to cells and methods for producing lipids using cellulosic carbon source. More specifically the invention relates to oleaginous bacterial cells, wherein genes encoding at least one cellulolytic activity has been introduced. This invention also relates to methods for lipid production by cultivating an oleaginous bacterial strain or strains capable of expressing one or more cellulolytic activity.
Claims22
An oleaginous bacterial cell that accumulates at least 10% (w/w) of its biomass dry weight as lipids after cultivation in conditions for lipid production, wherein genes encoding at least one endocellulase, at least one exocellulase, at least one cellobiase, and at least one ABC type transporter capable of facilitating cellobiose uptake operably linked to a suitable promoter sequence have been introduced.
An oleaginous bacterial cell that accumulates at least 10% (w/w) of its biomass dry weight as lipids after cultivation in conditions for lipid production, wherein genes encoding at least one endocellulase, at least one exocellulase, at least one cellobiase, and at least one ABC type transporter capable of facilitating cellobiose uptake operably linked to a suitable promoter sequence have been introduced, and
- wherein:
- (i) the endocellulase is a polypeptide comprising an amino acid sequence having at least 80% identity to SEQ ID NO:
- 1 (CenA), SEQ ID NO:
- 3 (CenB), SEQ ID NO:
- 5 (CenC) or SEQ ID NO:
- 11 (Ce16A);
- (ii) the exocellulase is a polypeptide comprising an amino acid sequence having at least 80% identity to SEQ ID NO:
- 9 (Cex) or SEQ ID NO:
- 7 (CbhA);
- (iii) the cellobiase is a polypeptide comprising an amino acid sequence having at least 80% identity to polypeptide SEQ ID NO:
- 17 (BglC);
- and
- (iv) the ABC type transporter is a polypeptide comprising an amino acid sequence having at least 80% identity to SEQ ID NO:
- 13 (Bg1A) or SEQ ID NO:
- 15 (Bg1B).
Dependencies are inferred from the claim text.