Biosensor incorporating protein-immobilized membrane and method of immobilizing protein in biosensor
AssigneeARKRAY, Inc.
InventorsKoji Katsuki, Hideaki Yamaoka
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Abstract
The present invention relates to a protein-immobilized membrane (14) including a cell membrane homologous layer (14A) and a protein (14B) immobilized to the cell membrane homologous layer (14A), where the protein contains cytochrome or a cytochrome complex. The present invention also relates to a method for forming a protein-immobilized membrane (14), and an enzyme-immobilized electrode and a biosensor (X1) provided with a protein-immobilized membrane (14). Preferably, the cell membrane homologous layer (14A) may contain a phospholipid polymer, and the protein (14B) may be CyGDH including an α subunit having a glucose dehydrogenase activity and cytochrome C having a function of electron transfer.
Claims21
A biosensor, comprising:
- a substrate;
- a capillary formed on the substrate for passage of a fluid to be analyzed;
- an electrode formed on the substrate;
- and
- a reagent portion formed on the substrate to cover the electrode;
- wherein the reagent portion comprises:
- an orientation-controlling layer formed on the electrode in contact therewith;
- and
- a layer of protein that is immobilized on a side of the orientation-controlling layer opposite the electrode in direct contact with the orientation-controlling layer, the layer of protein containing a cytochrome or a cytochrome complex, and the cytochrome or cytochrome complex of the layer of protein being oriented toward the orientation-controlling layer;
- and
- wherein the orientation-controlling layer contains a phospholipid polymer, the phospholipid polymer being 2-methacryloyloxyethyl phosphorylcholine polymer.
A biosensor, comprising:
- a substrate;
- a capillary formed on the substrate for passage of a fluid to be analyzed;
- an electrode formed on the substrate;
- and
- a reagent portion formed on the substrate to cover the electrode;
- wherein the reagent portion comprises:
- an orientation-controlling layer formed on the electrode in contact therewith;
- and
- a layer of protein that is immobilized on a side of the orientation-controlling layer opposite the electrode in direct contact with the orientation-controlling layer, the layer of protein containing a cytochrome or a cytochrome complex, and the cytochrome or cytochrome complex of the layer of protein being oriented toward the orientation-controlling layer;
- and
- wherein the orientation-controlling layer contains a silane coupling agent for bonding the orientation-controlling layer to the electrode.
A method for immobilizing a protein in a biosensor, the biosensor comprising:
- a substrate;
- a capillary formed on the substrate for passage of a fluid to be analyzed;
- and an electrode formed on the substrate;
- the method comprising:
- forming an orientation-controlling layer on a portion of the electrode in contact therewith;
- and
- causing a layer of protein to immobilize on a side of the orientation-controlling layer opposite the electrode in direct contact with the orientation-controlling layer, the layer of protein containing cytochrome or a cytochrome complex, the cytochrome or cytochrome complex of the layer of protein being oriented toward the orientation-controlling layer;
- and
- wherein the orientation-controlling layer contains a phospholipid polymer, the phospholipid polymer is 2-methacryloyloxyethyl phosphorylcholine polymer.
A method for immobilizing a protein in a biosensor, the biosensor comprising:
- a substrate;
- a capillary formed on the substrate for passage of a fluid to be analyzed;
- and an electrode formed on the substrate;
- the method comprising:
- forming an orientation-controlling layer on a portion of the electrode in contact therewith;
- and
- causing a layer of protein to immobilize on a side of the orientation-controlling layer opposite the electrode in direct contact with the orientation-controlling layer, the layer of protein containing cytochrome or a cytochrome complex, the cytochrome or cytochrome complex of the layer of protein being oriented toward the orientation-controlling layer;
- and
- wherein the orientation-controlling layer is formed to contain a silane coupling agent for bonding the orientation-controlling layer to the electrode.
Dependencies are inferred from the claim text.