Escherichia coli engineered for isobutyraldehyde production
AssigneeThe Regents of the University of California
InventorsShota Atsumi, Gabriel Rodriguez
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Abstract
The present disclosure provides recombinant E. coli and other bacteria with reduced alcohol dehydrogenase and/or aldehyde reductase activity. The present disclosure further provides recombinant E. coli and other bacteria with reduced isobutyraldehyde reductase activity. Methods for the production and the uses of the recombinant bacteria are also provided. Specifically, recombinant bacteria further expressing 2-keto-acid decarboxylase can be used for producing higher aldehydes or other non-alcohol chemicals derived from aldehydes.
Claims18
A genetically modified Escherichia coli (E. coli) comprising
- a recombinant nucleotide sequence encoding 2-keto-acid decarboxylase;
- a deletion in an eutE gene:
- and
- a mutation in each of genes set forth in group (a) or group (b):
- (a) adhE, yqhD, adhP, eutG, yiaY, and yjgB genes, wherein said mutation reduces alcohol dehydrogenase activity of products of said genes;
- or
- (b) adhE, yqhD, adhP, eutG, fucO, and yjgB genes, and wherein said mutation reduces isobutyraldehyde reductase activity of products of said genes.
Dependencies are inferred from the claim text.